Document Detail

Genetically engineered fluorescent voltage reporters.
MedLine Citation:
PMID:  22896802     Owner:  NLM     Status:  MEDLINE    
Fluorescent membrane voltage indicators that enable optical imaging of neuronal circuit operations in the living mammalian brain are powerful tools for biology and particularly neuroscience. Classical voltage-sensitive dyes, typically low molecular-weight organic compounds, have been in widespread use for decades but are limited by issues related to optical noise, the lack of generally applicable procedures that enable staining of specific cell populations, and difficulties in performing imaging experiments over days and weeks. Genetically encoded voltage indicators (GEVIs) represent a newer alternative that overcomes several of the limitations inherent to classical voltage-sensitive dyes. We critically review the fundamental concepts of this approach, the variety of available probes and their state of development.
Hiroki Mutoh; Walther Akemann; Thomas Knöpfel
Publication Detail:
Type:  Journal Article; Review     Date:  2012-06-06
Journal Detail:
Title:  ACS chemical neuroscience     Volume:  3     ISSN:  1948-7193     ISO Abbreviation:  ACS Chem Neurosci     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-16     Completed Date:  2013-04-10     Revised Date:  2013-08-18    
Medline Journal Info:
Nlm Unique ID:  101525337     Medline TA:  ACS Chem Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  585-92     Citation Subset:  IM    
Knöpfel lab for Neuronal Circuit Dynamics, RIKEN Brain Science Institute , 2-1 Hirosawa, Wako City, Saitama, 351-0198 Japan.
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MeSH Terms
Cardiovascular Physiological Phenomena
Drug Evaluation, Preclinical / methods
Fluorescent Antibody Technique / methods*
Fluorescent Dyes / chemistry*
Genetic Engineering / methods*
Luminescent Proteins
Voltage-Sensitive Dye Imaging / methods*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Luminescent Proteins

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