| Genetically controlled removal of "spore photoproduct" from deoxyribonucleic acid of ultraviolet-irradiated Bacillus subtilis spores. | |
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MedLine Citation:
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PMID: 4204907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous genetic analysis indicated that at least two genes determine the ultraviolet (UV) sensitivity of Bacillus subtilis spores. The present study shows that these genes independently control two distinguishable processes for removing UV-induced spore photoproduct (5-thyminyl-5,6-dihydrothymine, or TDHT) from spore deoxyribonucleic acid. The first, is a spore repair mechanism by which TDHT is removed rapidly without appearing in acid-soluble form. This mechanism, which is demonstrated in both UV-resistant and excision-deficient strains, operates to a certain extent during germination without requiring vegetative growth. The second, demonstrated in a mutant which lacks the first mechanism, removes TDHT relatively slowly and only if germinated spores are allowed to develop toward vegetative cells. The latter mechanism appears identical to excision-resynthesis repair, since the mutation abolishing it renders the irradiated vegetative cells incapable of removing cyclobutane-type pyrimidine dimers. Blocking either one of these mechanisms only slightly affects the UV sensitivity of spores, but blocking both prevents TDHT removal and gives high UV sensitivity. |
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Authors:
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N Munakata; C S Rupert |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 111 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1972 Jul |
Date Detail:
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Created Date: 1974-04-05 Completed Date: 1974-04-05 Revised Date: 2010-09-10 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 192-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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growth & development,
metabolism*,
radiation effects Cell Survival Chromatography, Paper DNA Repair DNA, Bacterial / metabolism*, radiation effects Genes* Mutation Radiation Effects Spores, Bacterial / growth & development, metabolism*, radiation effects Thymidine Thymine / biosynthesis* Tritium Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 10028-17-8/Tritium; 50-89-5/Thymidine; 65-71-4/Thymine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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