| Genetic variation in 16S-23S rDNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species. | |
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MedLine Citation:
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PMID: 11345528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The structural variation in 16S-23S rDNA internal transcribed spacer regions (ITS) among Bacteroides species was assessed by PCR amplification and sequencing analysis, and its possible use for molecular diagnosis of these species was evaluated. Ninety strains of the genus Bacteroides, including the species B. distasonis, B. eggerthii, B. fragilis, B. ovatus, B. thetaiotaomicron, B. uniformis and B. vulgatus, produced one to three ITS amplification products with sizes ranging from 615 to 810 bp. Some Bacteroides strains could be differentiated at species level on the basis of ITS amplification patterns and restriction fragment length polymorphism (RFLP) analysis using a four-nucleotide-recognizing enzyme, Msp I. The results of sequence analysis of ITS amplification products revealed genes for Ile-tRNA and Ala-tRNA in all strains tested. The nucleotide sequence, except for that in tRNA-coding regions, was highly variable and characteristic for each species, but a common sequence among B. fragilis, B. thetaiotaomicron and B. ovatus was observed. A digoxigenin-labeled oligonucleotide probe (named FOT1), which was designed from this conserved sequence, specifically hybridized to the ITS amplification products from B. fragilis, B. thetaiotaomicron and B. ovatus. These results suggest that the ITS region is a useful target for the development of rapid and accurate techniques for identification of Bacteroides species. |
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Authors:
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T Kuwahara; I Norimatsu; H Nakayama; S Akimoto; K Kataoka; H Arimochi; Y Ohnishi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology and immunology Volume: 45 ISSN: 0385-5600 ISO Abbreviation: Microbiol. Immunol. Publication Date: 2001 |
Date Detail:
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Created Date: 2001-05-09 Completed Date: 2001-09-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: Japan |
Other Details:
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Languages: eng Pagination: 191-9 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, School of Medicine, The University of Tokushima, Tokushima, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteroides
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genetics*,
isolation & purification Bacteroides Infections / microbiology* Base Sequence Cloning, Molecular DNA, Bacterial / analysis* DNA, Ribosomal Spacer / analysis* Genetic Variation Humans Molecular Sequence Data Polymorphism, Restriction Fragment Length RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 23S / genetics Sequence Alignment Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal Spacer; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 23S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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