Document Detail

Genetic variation at the goat hormone-sensitive lipase ( LIPE) gene and its association with milk yield and composition.
MedLine Citation:
PMID:  20380772     Owner:  NLM     Status:  MEDLINE    
Hormone-sensitive lipase (LIPE) plays a fundamental role in the regulation of energy balance by releasing free fatty acids from adipose triacylglycerol stores. These fatty acids can be subsequently transferred to other body compartments to be oxidized or employed in other biochemical reactions. This enzymic function is particularly important in lactating animals because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland. In the current study, we partially sequenced the goat LIPE gene in several individuals. In doing so, we identified two synonymous polymorphisms at exons 2 (c.327C>A>T, triallelic polymorphism) and 3 (c.558C>T). Moreover, we found a mis-sense polymorphism at exon 6 (c.1162G>T) that involves an alanine to serine substitution at position 388. Analysis with Polyphen and Panther softwares revealed that this amino acid replacement is expected to be neutral. Performance of an association analysis with a variety of milk traits revealed that goat LIPE genotype has highly suggestive effects on milk yield (P=0.0032) as well as on C18:3 n-6g (P=0.0051), trans-10 cis-12 CLA (P=0.007) and C12:0 (P=0.0084) milk contents. These associations are concordant with the preference of LIPE to selectively mobilize medium-chain and unsaturated fatty acids.
Ali Zidi; Víctor M Fernández-Cabanás; Juan Carrizosa; Jordi Jordana; Baltasar Urrutia; Oliva Polvillo; Pedro González-Redondo; David Gallardo; Marcel Amills; Juan M Serradilla
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of dairy research     Volume:  77     ISSN:  1469-7629     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  190-8     Citation Subset:  IM    
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
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MeSH Terms
Amino Acid Substitution / genetics
Base Sequence
Genetic Association Studies
Genetic Variation
Lactation* / genetics,  metabolism
Linoleic Acids, Conjugated* / analysis,  biosynthesis
Milk / chemistry*,  metabolism
Polymorphism, Genetic
Sterol Esterase / genetics*,  metabolism
Reg. No./Substance:
0/CLA fatty acid; 0/Linoleic Acids, Conjugated; EC Esterase

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