| Genetic variation at the goat hormone-sensitive lipase ( LIPE) gene and its association with milk yield and composition. | |
| | |
MedLine Citation:
|
PMID: 20380772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hormone-sensitive lipase (LIPE) plays a fundamental role in the regulation of energy balance by releasing free fatty acids from adipose triacylglycerol stores. These fatty acids can be subsequently transferred to other body compartments to be oxidized or employed in other biochemical reactions. This enzymic function is particularly important in lactating animals because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland. In the current study, we partially sequenced the goat LIPE gene in several individuals. In doing so, we identified two synonymous polymorphisms at exons 2 (c.327C>A>T, triallelic polymorphism) and 3 (c.558C>T). Moreover, we found a mis-sense polymorphism at exon 6 (c.1162G>T) that involves an alanine to serine substitution at position 388. Analysis with Polyphen and Panther softwares revealed that this amino acid replacement is expected to be neutral. Performance of an association analysis with a variety of milk traits revealed that goat LIPE genotype has highly suggestive effects on milk yield (P=0.0032) as well as on C18:3 n-6g (P=0.0051), trans-10 cis-12 CLA (P=0.007) and C12:0 (P=0.0084) milk contents. These associations are concordant with the preference of LIPE to selectively mobilize medium-chain and unsaturated fatty acids. |
| | |
Authors:
|
Ali Zidi; Víctor M Fernández-Cabanás; Juan Carrizosa; Jordi Jordana; Baltasar Urrutia; Oliva Polvillo; Pedro González-Redondo; David Gallardo; Marcel Amills; Juan M Serradilla |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of dairy research Volume: 77 ISSN: 1469-7629 ISO Abbreviation: J. Dairy Res. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-12 Completed Date: 2010-07-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: England |
Other Details:
|
Languages: eng Pagination: 190-8 Citation Subset: IM |
Affiliation:
|
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Substitution
/
genetics Animals Base Sequence Dairying Exons Female Genetic Association Studies Genetic Variation Genotype Goats Lactation* / genetics, metabolism Linoleic Acids, Conjugated* / analysis, biosynthesis Milk / chemistry*, metabolism Polymorphism, Genetic Sterol Esterase / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/CLA fatty acid; 0/Linoleic Acids, Conjugated; EC 3.1.1.13/Sterol Esterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Alcohol consumption as a risk factor for pneumonia: a systematic review and meta-analysis.
Next Document: Growth hormone alters lipid composition and increases the abundance of casein and lactalbumin mRNA i...