| Genetic variability in cysteine protease genes of Haemonchus contortus. | |
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MedLine Citation:
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PMID: 15180323 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To increase the existent genetic variability in cysteine proteases, a polymorphism study was performed in Haemonchus contortus by comparing 2 different strains of the parasite: North American (NA) and Spanish (SP) strains. For this purpose, the polymorphism of 5 previously reported genes (AC-1, AC-3, AC-4, AC-5 and GCP-7) were analysed by PCR-SSCP and sequencing procedures. Based on the SSCP results, a total of 20 different alleles were identified for the 5 loci assessed. Except locus AC-5, all the loci were polymorphic. Loci AC-1, AC-3, AC-4 and GCP-7 showed 5, 8, 2 and 4 alleles, respectively. The allelic frequencies ranged from 0.0070 to 0.8560 and were significantly different between strains. In addition, nucleotide diversity analyses showed a significant variation within and between strains. The variations in the nucleotide sequence of the different alleles were translated in some cases into changes in the amino acid sequence. Evidence of genetic variability in cysteine proteases from two different strains of H. contortus for the same set of genes had not been previously reported. |
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Authors:
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A Ruiz; J M Molina; A Njue; R K Prichard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Parasitology Volume: 128 ISSN: 0031-1820 ISO Abbreviation: Parasitology Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-06-07 Completed Date: 2004-08-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0401121 Medline TA: Parasitology Country: England |
Other Details:
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Languages: eng Pagination: 549-59 Citation Subset: IM |
Affiliation:
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Parasitology Unit, Department of Animal Pathology, Veterinary Faculty, University of Las Palmas de Gran Canaria, 35416 Arucas, Las Palmas, Spain. aruiz@dpat.ulpgc.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Amino Acid Sequence Animals Base Sequence Cysteine Endopeptidases / genetics* DNA, Helminth / chemistry, genetics Genetic Variation / genetics Haemonchus / enzymology*, genetics* Male Molecular Sequence Data North America Phylogeny Polymerase Chain Reaction Polymorphism, Single Nucleotide / genetics Polymorphism, Single-Stranded Conformational Sequence Alignment Spain |
| Chemical | |
Reg. No./Substance:
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0/DNA, Helminth; EC 3.4.22.-/Cysteine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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