Document Detail


Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis.
MedLine Citation:
PMID:  15466582     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shiga toxins Stx1 and Stx2 play a prominent role in the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections. Several variants of the stx(2) gene, encoding Stx2, have been described. In this study, we developed a PCR-restriction fragment length polymorphism system for typing stx(2) genes of STEC strains. The typing system discriminates eight described variants and allows the identification of new stx(2) variants and STEC isolates carrying multiple stx(2) genes. A phylogenetic tree, based on the nucleotide sequences of the toxin-encoding genes, demonstrates that stx(2) sequences with the same PvuII HaeIII HincII AccI type generally cluster together.
Authors:
Liesbet De Baets; Imme Van der Taelen; Marina De Filette; Denis Piérard; Lesley Allison; Henri De Greve; Jean-Pierre Hernalsteens; Hein Imberechts
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  70     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-06     Completed Date:  2004-12-15     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6309-14     Citation Subset:  IM    
Affiliation:
Dienst Algemene Bacteriologie, Centrum voor Onderzoek in Diergeneeskunde en Agrochemie, Groeselenberg 99, B-1180 Ukkel, Belgium. lideb@var.fgov.be.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques
Base Sequence
Cattle
DNA, Bacterial / genetics
Disease Outbreaks
Escherichia coli / classification*,  genetics*,  isolation & purification,  pathogenicity
Escherichia coli Infections / epidemiology,  microbiology
Genes, Bacterial
Genetic Variation
Humans
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Shiga Toxin 2 / genetics*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Shiga Toxin 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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