| Genetic typing of alpha1-antitrypsin by immunofixation electrophoresis, identification of subtypes of Pi M. | |
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MedLine Citation:
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PMID: 1107448 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prolonged electrophoresis in alkaline agarose gels, followed by immunofixation, is a valuable addition to acid starch gel electrophoresis and isoelectric focusing for the genetic phenotyping of alpha1-antitrypsin. This technique is helpful in clarifying certain variants and in ascertaining types in serum and amniotic fluid samples with secondary changes. In addition, heterogeneity may be detected within the variants found at pH 4.95, analogous to hemoglobin polymorphism. Two "new" variants, PiMLamb and PiMBaldwin, have been detected by a combination of immunofixation electrophoresis and acid starch gel electrophoresis. |
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Authors:
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A M Johnson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of laboratory and clinical medicine Volume: 87 ISSN: 0022-2143 ISO Abbreviation: J. Lab. Clin. Med. Publication Date: 1976 Jan |
Date Detail:
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Created Date: 1976-03-15 Completed Date: 1976-03-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0375375 Medline TA: J Lab Clin Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 152-63 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Genetic Techniques* Genetic Variation Humans Immunoelectrophoresis Pedigree Phenotype Polymorphism, Genetic alpha 1-Antitrypsin / analysis, classification* |
| Chemical | |
Reg. No./Substance:
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0/alpha 1-Antitrypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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