Document Detail


Genetic typing of alpha1-antitrypsin by immunofixation electrophoresis, identification of subtypes of Pi M.
MedLine Citation:
PMID:  1107448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prolonged electrophoresis in alkaline agarose gels, followed by immunofixation, is a valuable addition to acid starch gel electrophoresis and isoelectric focusing for the genetic phenotyping of alpha1-antitrypsin. This technique is helpful in clarifying certain variants and in ascertaining types in serum and amniotic fluid samples with secondary changes. In addition, heterogeneity may be detected within the variants found at pH 4.95, analogous to hemoglobin polymorphism. Two "new" variants, PiMLamb and PiMBaldwin, have been detected by a combination of immunofixation electrophoresis and acid starch gel electrophoresis.
Authors:
A M Johnson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  87     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1976 Jan 
Date Detail:
Created Date:  1976-03-15     Completed Date:  1976-03-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  152-63     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Genetic Techniques*
Genetic Variation
Humans
Immunoelectrophoresis
Pedigree
Phenotype
Polymorphism, Genetic
alpha 1-Antitrypsin / analysis,  classification*
Chemical
Reg. No./Substance:
0/alpha 1-Antitrypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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