Document Detail


Genetic studies of the role of fatty acid and coenzyme A in photocatalytic inactivation of Escherichia coli.
MedLine Citation:
PMID:  22691444     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The roles of bacterial cellular components, namely, fatty acid profile and coenzyme A, in photocatalytic inactivation of bacteria were investigated. Escherichia coli BW25113, as a "parental strain", and its isogenic single-gene deletion mutants E. coli JW1081 (fabF(-) mutant) and E. coli JW3942 (coaA(-) mutant) showed different susceptibilities towards photocatalytic inactivation by titanium dioxide (TiO(2), irradiated by UVA lamps (λ = 365 nm)). Regulating the fatty acid composition through pre-incubation temperature adjustment demonstrated the crucial role of cell membrane fatty acid profile in bacterial susceptibility towards photocatalytic inactivation, while the lower coenzyme A level in coaA(-) mutant correlated well with its lower susceptibility towards photocatalytic inactivation. Furthermore, transmission electron microscopic study demonstrated the photocatalytic destruction process of bacterial cells. This is the first study using single-gene deletion mutants to explore better understanding of the photocatalytic inactivation mechanism of E. coli.
Authors:
Minghui Gao; Taicheng An; Guiying Li; Xin Nie; Ho-Yin Yip; Huijun Zhao; Po-Keung Wong
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-27
Journal Detail:
Title:  Water research     Volume:  -     ISSN:  1879-2448     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-6-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0105072     Medline TA:  Water Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
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