| Genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster in Lactococcus lactis MG1363. | |
MedLine Citation:
|
PMID: 16917516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In a recent proteomic analysis, we showed the overproduction of the ArcA and ArcB proteins in Lactococcus lactis MG1363 at low pH. The corresponding genes belong to the arcABD1C1C2TD2 cluster that encodes components of the arginine deiminase pathway. In this study, we characterized this cluster at the genetic level. Northern blot experiments showed the expression of at least seven transcripts, all induced by acidity. Transcript analysis using 5'RACE PCR (rapid amplification of cDNA ends polymerase chain reaction) in the arcB-arcD1 intergenic region. In silico analysis identified nine stem-loop structures, all located in intergenic regions. Collectively, these data suggest a role for RNA processing and (or) premature termination in the differential expression of genes within the arcABD1C1C2TD2 cluster. |
Authors:
|
Aurélie Budin-Verneuil; Emmanuelle Maguin; Yanick Auffray; Dusko S Ehrlich; Vianney Pichereau |
Related Documents
:
|
2675976 - Requirements for efficient in vitro transcription and translation: a study using yeast ... 1398126 - The nitrate reductase-encoding gene of volvox carteri: map location, sequence and induc... 23677676 - Complete genome sequence of a polyvalent bacteriophage, phikp26, active on salmonella a... 10844696 - Antisense rna-dependent transcription termination sites that modulate lysogenic develop... 17347866 - Molecular cloning and comparison of avian preproghrelin genes. 333396 - A comparison of transcriptional linkage of trna cistrons in yeast and e. coli by the ul... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Canadian journal of microbiology Volume: 52 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2006 Jul |
Date Detail:
|
Created Date: 2006-08-18 Completed Date: 2006-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
|
Languages: eng Pagination: 617-22 Citation Subset: IM |
Affiliation:
|
Laboratoire de microbiologie de l'environment, USC Institut national de la recherche agronomique (INRA), Université de Caen, Cedex, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins
/
genetics*,
metabolism Gene Expression Regulation, Bacterial / genetics* Hydrogen-Ion Concentration Hydrolases / genetics*, metabolism Lactococcus lactis / enzymology, genetics* Multigene Family Operon / genetics* RNA, Ribosomal / genetics*, metabolism Transcription, Genetic / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/RNA, Ribosomal; EC 3.-/Hydrolases; EC 3.5.3.6/arginine deiminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of glutathione in the growth of Bradyrhizobium sp. (peanut microsymbiont) under different envir...
Next Document: Transformation of Metarhizium anisopliae mediated by Agrobacterium tumefaciens.