Document Detail

Genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster in Lactococcus lactis MG1363.
MedLine Citation:
PMID:  16917516     Owner:  NLM     Status:  MEDLINE    
In a recent proteomic analysis, we showed the overproduction of the ArcA and ArcB proteins in Lactococcus lactis MG1363 at low pH. The corresponding genes belong to the arcABD1C1C2TD2 cluster that encodes components of the arginine deiminase pathway. In this study, we characterized this cluster at the genetic level. Northern blot experiments showed the expression of at least seven transcripts, all induced by acidity. Transcript analysis using 5'RACE PCR (rapid amplification of cDNA ends polymerase chain reaction) in the arcB-arcD1 intergenic region. In silico analysis identified nine stem-loop structures, all located in intergenic regions. Collectively, these data suggest a role for RNA processing and (or) premature termination in the differential expression of genes within the arcABD1C1C2TD2 cluster.
Aurélie Budin-Verneuil; Emmanuelle Maguin; Yanick Auffray; Dusko S Ehrlich; Vianney Pichereau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  52     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  617-22     Citation Subset:  IM    
Laboratoire de microbiologie de l'environment, USC Institut national de la recherche agronomique (INRA), Université de Caen, Cedex, France.
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MeSH Terms
Bacterial Proteins / genetics*,  metabolism
Gene Expression Regulation, Bacterial / genetics*
Hydrogen-Ion Concentration
Hydrolases / genetics*,  metabolism
Lactococcus lactis / enzymology,  genetics*
Multigene Family
Operon / genetics*
RNA, Ribosomal / genetics*,  metabolism
Transcription, Genetic / physiology*
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Ribosomal; EC 3.-/Hydrolases; EC deiminase

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