Document Detail


Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers.
MedLine Citation:
PMID:  15372197     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Almond shoots produced by axillary branching from clone VII derived from a seedling of cultivar Boa Casta were evaluated for somaclonal variation using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) analysis. To verify genetic stability we compared RAPD and ISSR patterns of plantlets obtained after 4 and 6 years of in vitro multiplication. A total of 64 RAPD and 10 ISSR primers gave 326 distinct and reproducible band classes, monomorphic across all 22 plantlets analysed. Thus, a total of 7,172 bands were generated, exhibiting homogeneous RAPD and ISSR patterns for the plantlets tested. These results suggest that the culture conditions used for axillary branching proliferation are appropriate for clonal propagation of almond clone VII, as they do not seem to interfere with the integrity of the regenerated plantlets. These results allowed us to establish the use of axillary branching plantlets (mother-plants) as internal controls for the analysis of somaclonal variation of shoots regenerated from other in vitro culture processes performed with clone VII (adventitious regeneration, regeneration from meristem culture, virus sanitation programs and genetic engineering).
Authors:
M Martins; D Sarmento; M M Oliveira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-15
Journal Detail:
Title:  Plant cell reports     Volume:  23     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-10-17     Completed Date:  2005-12-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  492-6     Citation Subset:  IM    
Affiliation:
IBET/ITQB, Quinta do Marquês, 2784-505, Oeiras, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Agriculture / methods
Clone Cells / metabolism
DNA, Plant / genetics*
Gene Expression Regulation, Plant / genetics
Genetic Engineering / methods
Genetic Markers / genetics*
Genetic Variation / genetics*
Genome, Plant
Molecular Biology / methods
Plant Shoots / genetics*,  growth & development,  metabolism
Polymorphism, Genetic / genetics
Prunus / genetics*,  growth & development,  metabolism
Regeneration / genetics
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Genetic Markers

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