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Genetic selection for active E.coli amber tRNA(Asn) exclusively led to glutamine inserting suppressors.
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MedLine Citation:
PMID:  7708493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Suppressor tRNAs are useful tools for determining identity elements which define recognition of tRNAs in vivo by their cognate aminoacyl-tRNA synthetases. This study was aimed at the isolation of active amber tRNA(Asn). Nineteen mutated tRNA(Asn)CUA having amber suppressor activity were selected by an in vivo genetic screen, and all exclusively inserted glutamine. From analysis of the different mutations it is concluded that glutamine accepting activity was obtained upon reducing the interaction strength between the first base pair of the tRNA(Asn)CUA by direct or indirect effects. Failure to isolate tRNA(Asn)CUA suppressors charged with asparagine as well as other evolutionary related amino acids is discussed.
Authors:
F Martin; G Eriani; J Reinbolt; G Dirheimer; J Gangloff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  23     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-05-05     Completed Date:  1995-05-05     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  779-84     Citation Subset:  IM    
Affiliation:
Unité Propre de Recherche 9002 du Centre National de la Recherche Scientifique, Strasbourg, France.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Escherichia coli / genetics*
Genes, Suppressor*
Glutamine / genetics*
Molecular Sequence Data
Mutagenesis, Insertional
RNA, Transfer, Asn / genetics*
Chemical
Reg. No./Substance:
0/RNA, Transfer, Asn; 56-85-9/Glutamine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 3 Year: 1995
Volume: 23 Issue: 5
First Page: 779 Last Page: 784
ID: 306759
PubMed Id: 7708493
Publisher Item Identifier: 4w0218

Genetic selection for active E.coli amber tRNA(Asn) exclusively led to glutamine inserting suppressors.
F Martin
G Eriani
J Reinbolt
G Dirheimer
J Gangloff
Unité Propre de Recherche 9002 du Centre National de la Recherche Scientifique, Strasbourg, France.



Article Categories:
  • Research Article


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