Document Detail


Genetic restriction and specificity of the immune response in mice to fusion proteins containing repeated sequences of the Plasmodium falciparum antigen Pf155/RESA.
MedLine Citation:
PMID:  7751017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genetic restriction and specificity of the immune response in mice to two fusion proteins, ZZ-M3 and ZZ-M5, were studied. These proteins contain two IgG-binding domains (ZZ) from staphylococcal protein A, and repeated sequences from the C-terminal [(VEHDAEEN)5 (VEEN)10] (M3) or central [(VEEPTVADDEH)3(VEEPTVAEEH)2] (M5) regions of the Plasmodium falciparum malaria blood stage antigen Pf155/RESA. Strong antibody and T-cell responses to M3 and M5 were linked to expression of the I-Ak allele, and T-cell responses to the bacterial fusion partner ZZ were restricted to mice of the H-2k haplotype. The response to M5 was less restricted than that to M3, giving intermediate responses in mice of H-2d haplotypes as well. However, ZZ-M5-primed lymph node (LN) cells from these mice were primarily induced to proliferate in vitro by the complete ZZ-M5 construct and not by synthetic peptides representing the repeated subunits in M5. The reactivity with intact Pf155/RESA in erythrocyte membrane immunofluorescence was weak of antisera from mice immunized with ZZ-M5, whereas the reactivity of antisera from mice immunized with ZZ-M3 roughly paralleled their reactivity with M3 in an enzyme-linked immunosorbent assay (ELISA). The antibody responses induced by immunization with ZZ-M3 or ZZ-M5 were specific for M3 or M5, respectively, while activated T cells displayed cross-reactivity between M3 and M5 in an in vitro proliferation assay. The results indicate that the assembly of repeated sequences in fusion proteins affects both the MHC class II restriction and the specificity of the induced antibody and T-cell responses.
Authors:
A Sjölander; R Andersson; M Hansson; K Berzins; P Perlmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  84     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-06-19     Completed Date:  1995-06-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  360-6     Citation Subset:  IM    
Affiliation:
Department of Immunology, Stockholm University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Protozoan / biosynthesis*
Antibody Specificity
Antigens, Protozoan / immunology*
Cell Division / immunology
Female
Histocompatibility Antigens Class II / immunology*
Mice
Mice, Inbred Strains
Molecular Sequence Data
Plasmodium falciparum / immunology*
Protozoan Proteins / immunology*
Recombinant Fusion Proteins / immunology
T-Lymphocytes / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Antigens, Protozoan; 0/Histocompatibility Antigens Class II; 0/Protozoan Proteins; 0/Recombinant Fusion Proteins
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