Document Detail

Genetic recombination in human melanoma and astrocytoma cell lines involves oncogenes and growth factor genes.
MedLine Citation:
PMID:  2293915     Owner:  NLM     Status:  MEDLINE    
We describe here the chromosomal distribution of sister chromatid exchanges (SCEs) in four human tumor cell lines (two melanomas and two astrocytomas), and have mapped the sister chromatid recombination (SCR) sites. A higher incidence of SCR sites than expected on the basis of chromosome length occurred in chromosomes 2, 4, 5 and 15 in both the RPMI 5966 and MEL57 melanoma cell lines, and in chromosomes 1, 5, 13 and 15 of the IJKt and GUVW astrocytoma cell lines. A majority of the recombination sites occurred close to chromosomal fragile sites. A third of these occurred at the same bands as fragile sites. The recombination sites involved the N-ras and the epidermal growth factor gene in the melanomas. In the astrocytomas, the N-ras, Rb and c-mos genes appeared to be involved in the recombination events. The beta 2-microglobulin gene was involved in both astrocytomas and one melanoma. The erbB2 was involved in SCR only in the RPMI melanoma.
M S Lakshmi; G V Sherbet
Related Documents :
6650505 - Regional localization for hla by recombination with a fragile site at 6p23.
3818945 - Effect of methotrexate on intracellular folate pools in purified myeloid precursor cell...
15637705 - Prevalence of the fmr1 mutation in taiwan assessed by large-scale screening of newborn ...
18586975 - Quantitative microbial community analysis of three different sulfidic mine tailing dump...
21271665 - Microdeletion of 17q22q23.2 encompassing tbx2 and tbx4 in a patient with congenital mic...
9730605 - Comparative fish mapping of mouse and rat homologues of twenty-five human x-linked genes.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  8     ISSN:  0262-0898     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:    1990 Jan-Feb
Date Detail:
Created Date:  1990-02-06     Completed Date:  1990-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  75-87     Citation Subset:  IM    
Division of Oncology, University of Newcastle upon Tyne, Medical School, U.K.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Astrocytoma / genetics*
Chromosome Fragile Sites
Chromosome Fragility
Chromosome Mapping
Chromosomes, Human, Pair 4
Growth Substances / genetics*
Melanoma / genetics*
Recombination, Genetic*
Sister Chromatid Exchange*
Tumor Cells, Cultured / physiology
Reg. No./Substance:
0/Growth Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Combination chemotherapy with cisplatin and nifedipine: synergistic antitumor effects against a cisp...
Next Document:  Limb lengthening by callotasis for children and adolescents. Early experience.