Document Detail


Genetic recombination in human melanoma and astrocytoma cell lines involves oncogenes and growth factor genes.
MedLine Citation:
PMID:  2293915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe here the chromosomal distribution of sister chromatid exchanges (SCEs) in four human tumor cell lines (two melanomas and two astrocytomas), and have mapped the sister chromatid recombination (SCR) sites. A higher incidence of SCR sites than expected on the basis of chromosome length occurred in chromosomes 2, 4, 5 and 15 in both the RPMI 5966 and MEL57 melanoma cell lines, and in chromosomes 1, 5, 13 and 15 of the IJKt and GUVW astrocytoma cell lines. A majority of the recombination sites occurred close to chromosomal fragile sites. A third of these occurred at the same bands as fragile sites. The recombination sites involved the N-ras and the epidermal growth factor gene in the melanomas. In the astrocytomas, the N-ras, Rb and c-mos genes appeared to be involved in the recombination events. The beta 2-microglobulin gene was involved in both astrocytomas and one melanoma. The erbB2 was involved in SCR only in the RPMI melanoma.
Authors:
M S Lakshmi; G V Sherbet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  8     ISSN:  0262-0898     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:    1990 Jan-Feb
Date Detail:
Created Date:  1990-02-06     Completed Date:  1990-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  75-87     Citation Subset:  IM    
Affiliation:
Division of Oncology, University of Newcastle upon Tyne, Medical School, U.K.
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MeSH Terms
Descriptor/Qualifier:
Astrocytoma / genetics*
Chromosome Fragile Sites
Chromosome Fragility
Chromosome Mapping
Chromosomes, Human, Pair 4
Genes*
Growth Substances / genetics*
Humans
Melanoma / genetics*
Oncogenes*
Recombination, Genetic*
Sister Chromatid Exchange*
Tumor Cells, Cultured / physiology
Chemical
Reg. No./Substance:
0/Growth Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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