Document Detail

Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung's disease.
MedLine Citation:
PMID:  17708610     Owner:  NLM     Status:  MEDLINE    
AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants. METHODS: Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar. RESULTS: The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (c2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes. CONCLUSION: There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.
Zhi-Qin Mao; Ying Huang; Mei Sun; Qiang Ruan; Ying Qi; Rong He; Yu-Jing Huang; Yan-Ping Ma; Yao-Hua Ji; Zheng-Rong Sun; Hong Gao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  13     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-21     Completed Date:  2007-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4350-4     Citation Subset:  IM    
Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China.
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MeSH Terms
Amino Acid Motifs
Amino Acid Sequence
Colon / metabolism*
Cytomegalovirus / genetics*
DNA, Viral / genetics,  metabolism*
Hirschsprung Disease / metabolism,  virology*
Infant, Newborn
Membrane Glycoproteins / analysis,  genetics*
Molecular Sequence Data
Polymorphism, Genetic / genetics*
Viral Proteins / analysis,  genetics*
Reg. No./Substance:
0/DNA, Viral; 0/Membrane Glycoproteins; 0/UL144 ORF protein, Human herpesvirus 5; 0/Viral Proteins

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