| Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung's disease. | |
| | |
MedLine Citation:
|
PMID: 17708610 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants. METHODS: Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar. RESULTS: The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (c2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes. CONCLUSION: There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD. |
| | |
Authors:
|
Zhi-Qin Mao; Ying Huang; Mei Sun; Qiang Ruan; Ying Qi; Rong He; Yu-Jing Huang; Yan-Ping Ma; Yao-Hua Ji; Zheng-Rong Sun; Hong Gao |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: World journal of gastroenterology : WJG Volume: 13 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-08-21 Completed Date: 2007-11-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
|
Languages: eng Pagination: 4350-4 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. m_zq2004@163.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Motifs Amino Acid Sequence Colon / metabolism* Cytomegalovirus / genetics* DNA, Viral / genetics, metabolism* Genotype Hirschsprung Disease / metabolism, virology* Humans Infant Infant, Newborn Membrane Glycoproteins / analysis, genetics* Molecular Sequence Data Phenotype Polymorphism, Genetic / genetics* Viral Proteins / analysis, genetics* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Viral; 0/Membrane Glycoproteins; 0/UL144 ORF protein, Human herpesvirus 5; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of serum interleukin-18 as a prognostic factor in patients with hepatocellular carcinoma.
Next Document: Association of high expression in rat gastric mucosal heat shock protein 70 induced by moxibustion p...