Document Detail

Genetic and physiological characterization of a formate-dependent 5'-phosphoribosyl-1-glycinamide transformylase activity in Bacillus subtilis.
MedLine Citation:
PMID:  8121396     Owner:  NLM     Status:  MEDLINE    
We have found that Bacillus subtilis possesses a second 5'-phosphoribosyl-1-glycinamide (GAR) transformylase catalysing the first one-carbon transfer reaction in the purine biosynthetic pathway. Inactivation of the purN gene encoding the N10-formyl tetrahydrofolate-dependent enzyme did not result in purine auxotrophy. However, growth of a purN strain was stimulated when either purine or formate was added to the growth medium. In cell-free extracts GAR could be formylated, provided formate was added to the assay mixture. From the purN strain, purine-requiring mutants were isolated. One of these mutant strains was defective in the formate-dependent formylation of GAR in vitro. The gene containing this second mutation was designated purT, and was mapped to approximately 20 degrees on the genetic map between the cysA and aroI markers.
H H Saxild; J H Jacobsen; P Nygaard
Related Documents :
7765396 - Evaluation of bacillus subtilis strain is53 for the production of bacillus anthracis pr...
18401746 - Isolation of novel alkaliphilic bacillus strains for cyclodextrin glucanotransferase pr...
18487406 - Occurrence and genetic diversity of bacillus anthracis strains isolated in an active wo...
10467056 - Resistance to bacillus thuringiensis cry1ac toxin in three strains of heliothis viresce...
9408576 - Reinforcement of pmma bone cement with a continuous wire coil--a canine femur study.
10379686 - Development of resistance and cross-resistance in pseudomonas aeruginosa exposed to sub...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  242     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-06     Completed Date:  1994-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  415-20     Citation Subset:  IM    
Department of Microbiology, Technical University of Denmark, Lyngby.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acyltransferases / genetics,  metabolism*
Bacillus subtilis / enzymology*,  genetics
Cloning, Molecular
Formic Acids / pharmacology
Genes, Bacterial*
Hydroxymethyl and Formyl Transferases*
Phosphoribosylglycinamide Formyltransferase
Transformation, Genetic
Reg. No./Substance:
0/Formic Acids; 64-18-6/formic acid; EC 2.1.2.-/Hydroxymethyl and Formyl Transferases; EC Formyltransferase; EC 2.3.-/Acyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Sequential gel mobility shift scanning of 5' upstream sequences of the Neurospora crassa am (GDH) ge...
Next Document:  Genetic and biochemical properties of an extracellular neutral metalloprotease from Staphylococcus h...