Document Detail


Genetic and physiological characterization of a formate-dependent 5'-phosphoribosyl-1-glycinamide transformylase activity in Bacillus subtilis.
MedLine Citation:
PMID:  8121396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have found that Bacillus subtilis possesses a second 5'-phosphoribosyl-1-glycinamide (GAR) transformylase catalysing the first one-carbon transfer reaction in the purine biosynthetic pathway. Inactivation of the purN gene encoding the N10-formyl tetrahydrofolate-dependent enzyme did not result in purine auxotrophy. However, growth of a purN strain was stimulated when either purine or formate was added to the growth medium. In cell-free extracts GAR could be formylated, provided formate was added to the assay mixture. From the purN strain, purine-requiring mutants were isolated. One of these mutant strains was defective in the formate-dependent formylation of GAR in vitro. The gene containing this second mutation was designated purT, and was mapped to approximately 20 degrees on the genetic map between the cysA and aroI markers.
Authors:
H H Saxild; J H Jacobsen; P Nygaard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  242     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-06     Completed Date:  1994-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  415-20     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Technical University of Denmark, Lyngby.
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / genetics,  metabolism*
Bacillus subtilis / enzymology*,  genetics
Cloning, Molecular
Formic Acids / pharmacology
Genes, Bacterial*
Hydroxymethyl and Formyl Transferases*
Mutation
Phosphoribosylglycinamide Formyltransferase
Transformation, Genetic
Chemical
Reg. No./Substance:
0/Formic Acids; 64-18-6/formic acid; EC 2.1.2.-/Hydroxymethyl and Formyl Transferases; EC 2.1.2.2/Phosphoribosylglycinamide Formyltransferase; EC 2.3.-/Acyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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