Document Detail

Genetic and physical maps of the Bacillus subtilis chromosome.
MedLine Citation:
PMID:  10101153     Owner:  NLM     Status:  MEDLINE    
Sequencing of the complete Bacillus subtilis chromosome revealed the presence of approximately 4100 genes, 1000 of which were previously identified and mapped by classical genetic crosses. Comparison of these experimentally determined positions to those derived from the nucleotide sequence showed discrepancies reaching up to 24 degrees (approximately 280 kb). The size of these discrepancies as a function of their position along the chromosome is not random but, apparently, reveals some periodicity. Our analyses demonstrate that the discrepancies can be accounted for by inaccurate positioning of the early reference markers with respect to which all subsequently identified loci were mapped by transduction and transformation. We conclude (i) that specific DNA sequences, such as recombination hotspots or presence of heterologous DNA, had no detectable effect on the results obtained by classical mapping, and (ii) that PBS1 transduction appears to be an accurate and unbiased mapping method in B. subtilis.
C Rivolta; M Pagni
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetics     Volume:  151     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-12     Completed Date:  1999-05-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1239-44     Citation Subset:  IM    
Institut de Génétique et de Biologie Microbiennes, Université de Lausanne, CH-1005 Lausanne, Switzerland.
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MeSH Terms
Bacillus Phages / genetics
Bacillus subtilis / genetics*,  virology
Chromosome Mapping*
Chromosomes, Bacterial / genetics
DNA, Bacterial / genetics
Genetic Markers
Genome, Bacterial
Models, Genetic
Physical Chromosome Mapping
Reproducibility of Results
Transduction, Genetic
Reg. No./Substance:
0/DNA, Bacterial; 0/Genetic Markers

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