Document Detail


Genetic optimization of recombinant glycoprotein production by mammalian cells.
MedLine Citation:
PMID:  10098276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetically modified mammalian cells are the preferred system for the production of recombinant therapeutic glycoproteins. Other applications include engineering of cell lines for drug screening and cell-based therapies, and the construction of recombinant viruses for gene therapy. This article highlights contemporary core genetic technologies and emerging strategies for genetically engineering mammalian cells for optimal recombinant-protein expression.
Authors:
M Fussenegger; J E Bailey; H Hauser; P P Mueller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Trends in biotechnology     Volume:  17     ISSN:  0167-7799     ISO Abbreviation:  Trends Biotechnol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-05-06     Completed Date:  1999-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8310903     Medline TA:  Trends Biotechnol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  35-42     Citation Subset:  IM    
Affiliation:
Institute of Biotechnology, ETH Hönggerberg, Zurich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics
Gene Expression Regulation
Genes / genetics
Glycoproteins / biosynthesis*
Glycosylation
Molecular Biology / methods*
Protein Engineering / methods*
Protein Processing, Post-Translational
Recombinant Proteins / biosynthesis,  isolation & purification
Recombination, Genetic
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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