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GENETIC MARKERS TO DISCRIMINATE BENIGN AND MALIGNANT THYROID NODULES WITH UNDETERMINED CYTOLOGY IN AN AREA OF BORDERLINE IODINE DEFICIENCY.
MedLine Citation:
PMID:  21979329     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: Fine needle aspiration (FNA) with cytologic evaluation is the most reliable tool for malignancy prediction in thyroid nodules, but cytologic diagnosis remains undetermined for 20 % of nodules. Aim: We investigated the diagnostic potential of a set of 6 marker genes to distinguish benign and malignant thyroid nodules. Subjects and Methods: The prospective study included 153 thyroid samples obtained by FNA of thyroid nodules from 151 patients (56 benign, 43 malignant and 54 nodules with undetermined cytology). Gene expression was evaluated by quantitative real-time PCR and statistical analysis of data was performed. All samples were analyzed for V600E BRAF mutation. Results: A decrease in TTF3 and HGD1 expression was observed in malignant nodules with respect to benign ones, while an increase in PLAB expression was demonstrated in these nodules. The decision model was valid for 88 of 99 cases of benign and malignant nodules, with a total of 11 false positive or negative predictions. The obtained malignant/benign phenotype prediction was also valid for 37 of 54 cases of nodules with undetermined cytology with a total of 8 false positive and 9 false negative predictions. V600E BRAF gene mutation was demonstrated in 19/43 malignant nodules, in 0/56 benign nodules and in 1/54 undetermined nodules. Conclusions: The expression profiles of three genes (TFF3, HGD1 and PLAB) allowed a good prediction for the differentiation of benign thyroid lesions and thyroid cancer starting from cells of fine needle aspiration; however this assay showed limitations when applied to discriminate thyroid nodules with undetermined cytology.
Authors:
M Tonacchera; P Agretti; T Rago; G De Marco; F Niccolai; A Molinaro; M Scutari; A Candelieri; D Conforti; R Musmanno; G Di Coscio; F Basolo; P Iacconi; P Miccoli; A Pinchera; P Vitti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-4
Journal Detail:
Title:  Journal of endocrinological investigation     Volume:  -     ISSN:  1720-8386     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806594     Medline TA:  J Endocrinol Invest     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Endocrinology, Research Center of Excellence AmbiSEN, University of Pisa, Pisa, Italy.
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