Document Detail


Genetic mapping of four dinucleotide repeat loci, DXS453, DXS458, DXS454, and DXS424, on the X chromosome using multiplex polymerase chain reaction.
MedLine Citation:
PMID:  1351869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dinucleotide CA repeat sequences in the human genome have been shown to be highly polymorphic due to variation in the length of the repeat-containing segment. Therefore, these markers can serve as anchor loci in the construction of a high-resolution genetic map of the human genome. In this study, we improved the efficiency of typing dinucleotide repeats using multiplex polymerase chain reaction (PCR). Dinucleotide repeat sequences of four previously identified markers (DXS453, DXS458, DXS454, and DXS424) on the long arm of the X chromosome were simultaneously amplified in a single PCR reaction. This multiplex PCR was applied to genotype individuals from the 40 CEPH reference families, and the genotypic data were used to determine the map position of the four loci with respect to eight reference markers in the Xq region by linkage analysis.
Authors:
T H Huang; R W Cottingham; D H Ledbetter; H Y Zoghbi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  13     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-07-30     Completed Date:  1992-07-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  375-80     Citation Subset:  IM    
Affiliation:
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping
Female
Genetic Markers*
Humans
Linkage (Genetics)
Male
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction / methods*
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid*
X Chromosome*
Grant Support
ID/Acronym/Agency:
MJT-009121/JT/NIH HHS
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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