Document Detail


Genetic manipulation in Sulfolobus islandicus and functional analysis of DNA repair genes.
MedLine Citation:
PMID:  23356319     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Recently, a novel gene-deletion method was developed for the crenarchaeal model Sulfolobus islandicus, which is a suitable tool for addressing gene essentiality in depth. Using this technique, we have investigated functions of putative DNA repair genes by constructing deletion mutants and studying their phenotype. We found that this archaeon may not encode a eukarya-type of NER (nucleotide excision repair) pathway because depleting each of the eukaryal NER homologues XPD, XPB and XPF did not impair the DNA repair capacity in their mutants. However, among seven homologous recombination proteins, including RadA, Hel308/Hjm, Rad50, Mre11, HerA, NurA and Hjc, only the Hjc nuclease is dispensable for cell viability. Sulfolobus encodes redundant BER (base excision repair) enzymes such as two uracil DNA glycosylases and two putative apurinic/apyrimidinic lyases, but inactivation of one of the redundant enzymes already impaired cell growth, highlighting their important roles in archaeal DNA repair. Systematically characterizing these mutants and generating mutants lacking two or more DNA repair genes will yield further insights into the genetic mechanisms of DNA repair in this model organism.
Authors:
Changyi Zhang; Bin Tian; Suming Li; Xiang Ao; Kevin Dalgaard; Serkan Gökce; Yunxiang Liang; Qunxin She
Related Documents :
9811479 - Biodistribution of radiolabeled lipid-dna complexes and dna in mice.
24730569 - Significance of the melanocortin 1 receptor in the dna damage response of human melanoc...
24699249 - Dna repair pathway selection caused by defects in tel1, sae2, and de novo telomere addi...
22958599 - Engineered cell-cell communication via dna messaging.
24285299 - Yy1 dna binding and interaction with yaf2 is essential for polycomb recruitment.
23385459 - Structure of the c-terminal half of human xpb helicase and the impact of the disease-ca...
15516599 - Cold sensitivity of thermophilic and mesophilic rna polymerases.
2169679 - Rapid detection of pseudorabies virus genomic sequences in biological samples from infe...
2157679 - Establishment of immortalized primate epithelial cells with sub-genomic ebv dna.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  41     ISSN:  1470-8752     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  405-10     Citation Subset:  IM    
Affiliation:
*State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Atypical protein kinases of the RIO family in archaea.
Next Document:  C/D box sRNA, CRISPR RNA and tRNA processing in an archaeon with a minimal fragmented genome.