| Genetic lesions associated with Muller's ratchet in an RNA virus. | |
| | |
MedLine Citation:
|
PMID: 8951375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The molecular basis of Muller's ratchet has been investigated using the important animal pathogen foot-and-mouth disease virus (FMDV). Clones from two FMDV populations were subjected to serial plaque transfers (repeated bottleneck events) on host BHK-21 cells. Relative fitness losses were documented in 11 out of 19 clones tested. Small fitness gains were observed in three clones. One viral clone attained an extremely low plating efficiency, suggesting that accumulation of deleterious mutations had driven the virus near extinction. Nucleotide sequence analysis revealed unique genetic lesions in multiply transferred clones that had never been seen in FMDVs isolated in nature or subjected to massive infections in cell culture. In particular, a frequent internal polyadenylate extension has identified a mutational hot spot on the FMDV genome. Furthermore, amino acid residue substitutions in internal capsid sites which are severely restricted during FMDV evolution, amounted to half of capsid replacements in the transferred clones. In addition, a striking dominance of non-synonymous replacements fixed upon large population infections of FMDV was not observed upon serial plaque transfers. The nucleotide sequence of the entire genome of a severely debilitated clone suggests that very few mutations may be sufficient to drive FMDV near extinction. The results provide an account of the molecular basis of Muller's ratchet for an RNA virus, and insight into the types of genetic variants which populate the mutant spectra of FMDV quasispecies. |
| | |
Authors:
|
C Escarmís; M Dávila; N Charpentier; A Bracho; A Moya; E Domingo |
Related Documents
:
|
11580025 - Migratory costs and the evolution of egg size and number in introduced and indigenous s... 20580115 - Evolutionary genetics in wild primates: combining genetic approaches with field studies... 15930815 - A perspective on the diversity of human adaptability. 9217155 - Evolution of genetic redundancy. 6790335 - Genetic dissection of segregation distortion. iii. unequal recovery of reciprocal recom... 2714645 - Correlation between genetic distances based on single loci and on skeletal morphology i... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of molecular biology Volume: 264 ISSN: 0022-2836 ISO Abbreviation: J. Mol. Biol. Publication Date: 1996 Nov |
Date Detail:
|
Created Date: 1997-01-02 Completed Date: 1997-01-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985088R Medline TA: J Mol Biol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 255-67 Citation Subset: IM |
Affiliation:
|
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aphthovirus / genetics* Cell Line Cricetinae Extinction, Psychological Mutation Nucleic Acid Conformation Plaque Assay RNA, Viral / analysis* |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: HIV-1 reverse transcriptase discriminates against non-self tRNA primers.
Next Document: Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by ...