Document Detail


Genetic instability and divergence of clonal populations in colon cancer cells in vitro.
MedLine Citation:
PMID:  16551697     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The accumulation of multiple chromosomal abnormalities is a characteristic of the majority of colorectal cancers and has been attributed to an underlying chromosomal instability. Genetic instability is considered to have a key role in the generation of genetic and phenotypic heterogeneity in cancer cells. To shed light on the dynamics of chromosomal instability in colon cancer cells, we have analyzed genetic divergence in clonal and subclonal derivates of chromosomally unstable (SW480) and stable (HCT116, LoVo) cell lines. Conventional G-banding karyotyping and arbitrarily primed PCR (AP-PCR) fingerprinting were used to calculate genetic distances among clones and parental cells, and to trace tree-type phylogenies among individual cells and clonal cell populations. SW480 cells showed enhanced karyotypic heterogeneity in clones as compared with parental cells. Moreover, genetic clonal divergence was also increased after two consecutive episodes of single-cell cloning, demonstrating that the homogeneity induced by the bottleneck of cloning is disrupted by genetic instability during clonal expansion and, as a consequence, heterogeneity is restored. These results demonstrate genetic drift in clonal populations originated from isolated cells. The generated cell heterogeneity coupled with selection provides the grounds for the reported feasibility of pre-neoplastic and neoplastic cells to generate new phenotypic variants with increased evolutionary potential.
Authors:
Laia Masramon; Elisenda Vendrell; Gemma Tarafa; Gabriel Capellà; Rosa Miró; Maria Ribas; Miguel A Peinado
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-21
Journal Detail:
Title:  Journal of cell science     Volume:  119     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-06     Completed Date:  2006-08-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1477-82     Citation Subset:  IM    
Affiliation:
IDIBELL-Institut de Recerca Oncològica, L'Hospitalet, 08907 Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Chromosomal Instability*
Chromosome Banding
Clone Cells
Colonic Neoplasms / genetics*
DNA Fingerprinting
Disease Progression
Genetic Drift
Genetic Heterogeneity*
Genetic Variation*
Humans
Phylogeny
Polymerase Chain Reaction

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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