Document Detail

Genetic and immunological characterisation of a multixenobiotic resistance system in the turbot (Scophthalmus maximus).
MedLine Citation:
PMID:  12031473     Owner:  NLM     Status:  MEDLINE    
Pleiotropic resistance driven by transport proteins constitutes a very ubiquitous protection mechanism against natural or synthetic toxic compounds. The multidrug (MDR) or multixenobiotic (MXR) system has been identified in many different species, and may be used as a biomarker for pollution assessment. Here we report the existence of a gene encoding a MXR-related protein in a benthic fish species, the turbot Scophthalmus maximus, and its constitutive expression in several tissues. A 433bp cDNA fragment has been cloned by RT-PCR. The deduced amino-acid sequence shares close to 80% homology with class I or class II mammalian MDR proteins. This cDNA corresponds to a major mRNA of 5.6 kb and encodes a protein having an apparent molecular weight of 83 kDa. Constitutive expression levels assessed by semi-quantitative RT-PCR and Western blot, revealed that the kidney and the brain, and to a lesser extent, the heart, gills and intestine, are the organs which contain the highest amount of both MXR mRNAs or proteins. This tissue specific expression suggests a role for the identified mechanism in protection against endogenous or exogenous toxic compounds.
Renaud Tutundjian; J Cachot; F Leboulenger; C Minier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  132     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-28     Completed Date:  2003-01-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  463-71     Citation Subset:  IM    
Laboratoire d'Ecotoxicologie, UPRES EA 3222, Université du Havre, 25 rue Philippe Lebon, BP 540, 76058 Le Havre, France.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
Drug Resistance, Multiple / genetics*,  immunology*
Fish Proteins / analysis,  chemistry,  genetics
Flatfishes / genetics*,  immunology*
Gene Expression Profiling
Genes, MDR / genetics*
Molecular Sequence Data
Molecular Weight
Organ Specificity
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Xenobiotics / pharmacology*
Reg. No./Substance:
0/DNA, Complementary; 0/Fish Proteins; 0/RNA, Messenger; 0/Xenobiotics

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