Document Detail


Genetic heterogeneity and efficiency of two different methods of adenovirus-mediated gene transfer in a rat liver transplantation model.
MedLine Citation:
PMID:  16554995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We used recombinant adenoviral vectors for gene therapy in liver transplantation, and investigated the efficacy of gene transfer and expression on the grafts and genetic heterogeneity, with two exogenous gene transfer methods in three different syngeneic rat strains. METHODS: We transferred adenoviral vector encoding Escherichia coli beta-galactosidase via a donor tail vein 3 days before transplantation; via a recipient tail vein immediately after grafting; and ex vivo by perfusion and clamping during transplantation. RESULTS: The high efficacy of beta-galactosidase gene transfer and expression was seen in both delivery systems, with 70% positivity for hepatocytes on day 3, which persisted for at least 3 weeks after transplantation. The efficacy of gene transfer and expression was similar in the three strains (DA, Lewis, and PVG). CONCLUSIONS: These data suggest that adenovirus-mediated gene transfer delivers effective gene therapy by tail vein injection of a donor or a recipient, or by ex vivo graft perfusion in rat liver transplantation. It is not necessary to consider the differences in the strains. Furthermore, ex vivo graft perfusion is probably more suitable not only for rat liver transplantation but also possibly for future clinical application.
Authors:
Kensuke Adachi; Masayuki Fujino; Yusuke Kitazawa; Naoko Funeshima; Xiao-Kang Li
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery today     Volume:  36     ISSN:  0941-1291     ISO Abbreviation:  Surg. Today     Publication Date:  2006  
Date Detail:
Created Date:  2006-03-23     Completed Date:  2006-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204360     Medline TA:  Surg Today     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  367-75     Citation Subset:  IM    
Affiliation:
Laboratory of Transplantation Immunology, Department of Innovative Surgery, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae*
Animals
Escherichia coli / genetics*
Gene Therapy / methods*
Gene Transfer Techniques*
Genetic Heterogeneity
Liver Transplantation / methods*
Rats
Rats, Inbred Lew
Recombinant Proteins / therapeutic use*
beta-Galactosidase / genetics*
Chemical
Reg. No./Substance:
0/Recombinant Proteins; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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