| Genetic evidence for the creation of a reinitiation site by mutation inside the yeast ura 2 gene. | |
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MedLine Citation:
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PMID: 381838 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ura 2 gene of yeast codes for two enzymatic activities which are translated from a unique messenger RNA in the order carbamoyl-phosphate synthetase (CPSase), aspartate transcarbamylase (ATCase) (Lacroute, 1968; Denis-Duphil and Kaplan, 1976). Nonsense mutations in the CPSPase region cause a complete loss in ATCase activity by a total polar effect, characteristic of eukaryotic mRNA translation, and due to the unique site of protein initiation present on each messenger (Shaffer et al., 1969). A triple nonsense mutant in the CPSase has been constructed by recombination and ATCase+ revertants have been selected from it. Among seventeen revertants obtained, three had a deletion covering the three nonsense mutations relieving thus the polar effect (Fink and Styles, 1974) but fourteen others examined had retained all the CPSase DNA including the three nonsense mutations; this can be explained in the present state of knowledge only by the creation by mutation of reinitiation site either for transcription or for translation in the region of the ura 2 gene distal to the last nonsense mutation. |
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Authors:
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F Exinger; F Lacroute |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular & general genetics : MGG Volume: 173 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1979 May |
Date Detail:
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Created Date: 1979-10-24 Completed Date: 1979-10-24 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 109-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspartate Carbamoyltransferase
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genetics* Mutation Peptide Chain Initiation, Translational* RNA, Messenger / genetics Recombination, Genetic Saccharomyces cerevisiae / genetics* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 2.1.3.2/Aspartate Carbamoyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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