Document Detail

Genetic and epigenetic instability of amplification-prone sequences of a novel B chromosome induced by tissue culture in Plantago lagopus L.
MedLine Citation:
PMID:  19847437     Owner:  NLM     Status:  MEDLINE    
Gene amplification is prevalent in many eukaryotes and has been found linked to various phenomena such as ontogenesis, carcinogenesis, in vitro culturing, neoplasia and drug resistance. Earlier, we reported a novel B chromosome in Plantago lagopus L., which was found to have arisen as a result of massive amplification of 5S rDNA. In addition, the chromosome is also composed of 45S rDNA and transposable elements. While the importance of gene amplification cannot be underestimated, its mechanism of origin is still unclear. Therefore, the aim of the present study was to determine whether amplification can be reactivated in the novel B chromosome. For this purpose, in vitro culture was used as stress. Three modes of tissue culture, i.e., direct, indirect and somatic embryogenesis were used for raising in vitro cultures. The variations due to genetic and epigenetic mechanisms were assessed in regenerants using molecular techniques, namely, PCR-RFLP, SSAP and MSAP. The retrotransposon-based molecular markers were applied to detect the polymorphism within transposable elements of in vitro regenerated and mother plants. We detected the variations that may be due to genetic changes either because of element recombination or activation of transposable elements which can lead to increase in the copy number. MSAP analysis revealed the differences in the DNA methylation pattern of the regenerants derived from novel chromosome bearing mother plants. Some regenerated plants were associated with increase and decrease in DNA methylation of both internal and external cytosine of the CCGG sequence.
Gurmeet Kour; Balbir Kour; Sanjana Kaul; Manoj Kumar Dhar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-22
Journal Detail:
Title:  Plant cell reports     Volume:  28     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-03-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1857-67     Citation Subset:  IM    
Plant Genomics Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, India.
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MeSH Terms
Base Sequence
Chromosomes, Plant / genetics*
Culture Media
DNA Methylation / drug effects,  genetics
DNA, Intergenic / genetics
DNA, Ribosomal / genetics
Epigenesis, Genetic* / drug effects
Gene Amplification / genetics*
Genomic Instability / drug effects,  genetics*
Plant Growth Regulators / pharmacology
Plant Leaves / drug effects,  growth & development
Plant Shoots / drug effects,  growth & development
Plantago / drug effects,  genetics*,  physiology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Regeneration / drug effects,  genetics
Tissue Culture Techniques / methods*
Reg. No./Substance:
0/Culture Media; 0/DNA, Intergenic; 0/DNA, Ribosomal; 0/Plant Growth Regulators

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