Document Detail


Genetic and epigenetic characteristics of gastric cancers with JC virus T-antigen.
MedLine Citation:
PMID:  19938198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To clarify the significance of JC virus (JCV) T-antigen (T-Ag) expression in human gastric cancer. METHODS: We investigated the relationship between T-Ag detected by immunohistochemistry and Epstein-Barr virus (EBV) infection, microsatellite instability (MSI), and genetic and epigenetic alterations in gastric cancers. Mutations in the p53, beta-catenin, KRAS, BRAF, PIK3CA genes were analyzed by polymerase chain reaction (PCR)-single strand conformation polymorphism and DNA sequencing. Allelic losses were determined by PCR at 7 microsatellite loci. Aberrant DNA methylation was analyzed by MethyLight assay. RESULTS: JCV T-Ag protein expression was found in 49% of 90 gastric cancer tissues. T-Ag positivity was not correlated with clinicopathological characteristics. T-Ag expression was detected in a similar percentage of EBV positive cancers (4 of 9, 44%) and EBV negative cancers (35 of 73, 48%). T-Ag expression was detected in a significantly lower percentage of MSI-H cancers (14%) than in non MSI-H cancers (55%, P = 0.005). T-Ag expression was detected in a significantly higher percentage of cancers with nuclear/cytoplasmic localization of beta-catenin (15 of 21, 71%) than in cancers without (42%, P = 0.018). p53 mutations were detected in a significantly lower percentage of T-Ag positive cancers (32%) than in T-Ag negative cancers (57%, P = 0.018). T-Ag positive gastric cancers showed a significant increase in the allelic losses and aberrant methylation compared with T-Ag negative gastric cancers (P = 0.008 and P = 0.003). CONCLUSION: The results suggest that JCV T-Ag is involved in gastric carcinogenesis through multiple mechanisms of genetic and epigenetic alterations.
Authors:
Satoshi Yamaoka; Hiroyuki Yamamoto; Katsuhiko Nosho; Hiroaki Taniguchi; Yasushi Adachi; Shigeru Sasaki; Yoshiaki Arimura; Kohzoh Imai; Yasuhisa Shinomura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  15     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2010-02-24     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  5579-85     Citation Subset:  IM    
Affiliation:
First Department of Internal Medicine, Sapporo Medical University, Chuo-ku, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*,  immunology*
Aged
Alleles
Antigens, Viral, Tumor / metabolism*
Epigenesis, Genetic*
Female
Humans
Immunohistochemistry / methods
JC Virus / metabolism*
Loss of Heterozygosity
Male
Microsatellite Repeats / genetics
Middle Aged
Mutation
Sequence Analysis, DNA
Stomach Neoplasms / genetics*,  immunology*
Chemical
Reg. No./Substance:
0/Antigens, Viral, Tumor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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