Document Detail


Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum.
MedLine Citation:
PMID:  20971205     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Renewable diesel surrogates made from shorter chain length fatty acids have improved cold flow properties. Acyl-ACP thioesterases specific for shorter chain length fatty acids are therefore of considerable interest in the genetic engineering of biofuel producing organisms, both for their ability to increase the production of shorter fatty acids, and for their involvement in fatty acid secretion in bacterial systems. Here we show that the heterologous expression of two thioesterases, biased towards the production of lauric (C12:0) and myristic acid (C14:0), causes increased accumulation of shorter chain length fatty acids in the eukaryotic microalga Phaeodactylum tricornutum. Accumulation of shorter chain length fatty acids corresponds to transgene transcript levels. We achieved levels of C12:0 of up to 6.2% of total fatty acids and C14:0 of up to 15% by weight. Unlike observations in cyanobacteria, no significant secretion of fatty acids was observed. Instead, we found that 75-90% of the shorter chain length fatty acids produced was incorporated into triacylglycerols. Our results demonstrate that overexpression of thioesterases is a valid way to improve the biofuel production phenotype of eukaryotic microalgae.
Authors:
Randor Radakovits; Patrick M Eduafo; Matthew C Posewitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-27
Journal Detail:
Title:  Metabolic engineering     Volume:  13     ISSN:  1096-7184     ISO Abbreviation:  Metab. Eng.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  89-95     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Chemistry and Geochemistry, Colorado School of Mines, 1500 Illinois St., Golden, CO 80401, USA.
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