Document Detail

Genetic elimination of eNOS reduces secondary complications of experimental subarachnoid hemorrhage.
MedLine Citation:
PMID:  23549379     Owner:  NLM     Status:  MEDLINE    
Delayed complications of subarachnoid hemorrhage (SAH) such as angiographic vasospasm, cortical spreading ischemia, microcirculatory dysfunction, and microthrombosis are reported in both patients and animal models of SAH. We demonstrated previously that SAH is associated with increased oxidative stress in the brain parenchyma, and that this correlates with dysfunction of endothelial nitric oxide synthase (eNOS) (homodimeric uncoupling). Uncoupling of eNOS exacerbated oxidative stress and enhanced nitric oxide (NO) depletion, and was associated with multiple secondary complications such as microthrombosis, neuronal apoptosis, and release of reactive oxygen species. Thus, we hypothesized that genetic abbrogation of eNOS would confer a beneficial effect on the brain after SAH. Using a prechiasmatic injection model of SAH, we show here that eNOS knockout (KO) significantly alleviates vasospasm of the middle cerebral artery and reduces superoxide production. Endothelial nitric oxide synthase KO also affected other nitric oxide synthase isoforms. It significantly increases neuron nitric oxide synthase expression but has no effect on inducible nitric oxide synthase. Endothelial nitric oxide synthase KO decreases Zn(2+) release after SAH, reduces microthrombi formation, and prevent neuronal degeneration. This work is consistent with our findings where, after SAH, increased oxidative stress can uncouple eNOS via Zn(2+) thiolate oxidation, or theoretically by depletion or oxidation of tetrahydrobiopterin, resulting in a paradoxical release of superoxide anion radical, further exacerbating oxidative stress and microvascular damage.
Mohammed Sabri; Jinglu Ai; Elliot Lass; Josephine D'abbondanza; R Loch Macdonald
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-04-03
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  33     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-01     Completed Date:  2013-09-06     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1008-14     Citation Subset:  IM    
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MeSH Terms
Blotting, Western
Cerebrovascular Circulation / physiology
Disease Models, Animal
Fibrinogen / metabolism
Intracranial Thrombosis / enzymology,  etiology,  pathology,  prevention & control*
Mice, Knockout
Neurons / enzymology,  pathology
Nitric Oxide / metabolism
Nitric Oxide Synthase Type III / genetics*,  physiology
Oxidative Stress / genetics,  physiology
Subarachnoid Hemorrhage / complications*,  enzymology
Superoxides / metabolism
Vasospasm, Intracranial / enzymology,  etiology,  pathology,  prevention & control*
Zinc / metabolism
Reg. No./Substance:
11062-77-4/Superoxides; 31C4KY9ESH/Nitric Oxide; 9001-32-5/Fibrinogen; EC Oxide Synthase Type III; EC protein, mouse; J41CSQ7QDS/Zinc

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