Document Detail

Genetic diversity of transmission blocking vaccine candidate (Pvs25 and Pvs28) antigen in Plasmodium vivax clinical isolates from Iran.
MedLine Citation:
PMID:  18950597     Owner:  NLM     Status:  MEDLINE    
The leading candidates for a Transmission Blocking Vaccine (TBV) in Plasmodium vivax parasite are the ookinete surface protein 25 (Pvs25) and Pvs28, which their phase I clinical trial is ongoing. Therefore, we carried out survey of polymorphisms of the pvs25 and pvs28 genes in P. vivax populations that are circulating in the two malaria areas of contrasting endemicity in Iran, before field application of the TBV. To characterize the polymorphisms of pvs25 and pvs28 genes, 50 isolates were analyzed by sequencing method and their gene structure was compared with parasite populations from India, Bangladesh, Indonesia, Thailand, Mexico and Brazil. Three mutations were detected in pvs25 and pvs28 including Q87K, E97Q, I130T and M52L, T65K, T140S with two and four distinct haplotypes, in comparison with the Sal I sequence type, respectively. Both haplotypes of Pvs25 were found among northern and southern P. vivax isolates; however, only two and three of the Pvs28 variants were observed among the northern and southern isolates, respectively. In conclusion, the present results show the limited sequence polymorphism of the pvs25 and pvs28 genes among field P. vivax population in Iran. These results highly encourage with respect to applicability of Pvs25 and Pvs28-based vaccine against P. vivax infection in the region, where these parasites are prevalent, whether these occur in the temperate or tropical zones.
Sedigheh Zakeri; Sepand Razavi; Navid Dinparast Djadid
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-30
Journal Detail:
Title:  Acta tropica     Volume:  109     ISSN:  1873-6254     ISO Abbreviation:  Acta Trop.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370374     Medline TA:  Acta Trop     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  176-80     Citation Subset:  IM    
Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU810766;  EU810767;  EU810768;  EU810769;  EU810770;  EU810771;  EU810772;  EU810773;  EU810774
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MeSH Terms
Amino Acid Sequence
Amino Acid Substitution / genetics
Antigens, Protozoan / chemistry,  genetics*
Antigens, Surface / chemistry,  genetics*
Clinical Trials as Topic
DNA, Protozoan / chemistry,  genetics
Genetic Variation*
Malaria Vaccines / chemistry,  genetics*,  immunology
Malaria, Vivax / immunology,  parasitology*
Molecular Sequence Data
Mutation, Missense
Plasmodium vivax / genetics*,  isolation & purification
Sequence Analysis, DNA
Sequence Homology
Reg. No./Substance:
0/Antigens, Protozoan; 0/Antigens, Surface; 0/DNA, Protozoan; 0/Malaria Vaccines; 0/Pvs25 protein, P vivax; 0/Pvs28 protein, P vivax

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