| Genetic diversity and diet specificity of ruminal Prevotella revealed by 16S rRNA gene-based analysis. | |
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MedLine Citation:
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PMID: 20158525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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16S rRNA gene-based analysis of rumen Prevotella was carried out to estimate the diversity and diet specificity of bacteria belonging to this genus. Total DNA was extracted from the rumen digesta of three sheep fed two diets with different hay-to-concentrate ratios (10 : 1 and 1 : 2). Real-time PCR quantification of Prevotella revealed that the relative abundance of this genus in the total rumen bacteria was up to 19.7%, while the representative species Prevotella bryantii and Prevotella ruminicola accounted for only 0.6% and 3.8%, respectively. Denaturing gradient gel electrophoresis analysis for Prevotella revealed shifts in the community composition with the diet. Analysis of 16S rRNA gene clone libraries showed significant differences (P=0.001) between clones detected from the sheep on the diets with different hay-to-concentrate ratios. The majority (87.8%) of Prevotella clones had <97% sequence similarity with known rumen Prevotella. These data suggest that uncultured Prevotella is more abundant than known Prevotella and that members of this genus appear to have specific metabolic niches. |
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Authors:
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Aschalew Z Bekele; Satoshi Koike; Yasuo Kobayashi |
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Publication Detail:
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Type: Journal Article Date: 2010-01-27 |
Journal Detail:
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Title: FEMS microbiology letters Volume: 305 ISSN: 1574-6968 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-05-07 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 49-57 Citation Subset: IM |
Affiliation:
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Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB519308; AB519309; AB519310; AB519311; AB519312; AB519313; AB519314; AB519315; AB519316; AB519317; AB519318; AB519319; AB519320; AB519321; AB519322; AB519323; AB519324; AB519325; AB519326; AB519327; AB519328; AB519329; AB519330; AB519331; AB519332; AB519333; AB519334; AB519335; AB519336; AB519337; AB519338; AB519339; AB519340; AB519341; AB519342; AB519343; AB519344; AB519345; AB519346; AB519347; AB519348; AB519349; AB519350; AB519351; AB519352; AB519353; AB519354; AB519355; AB519356; AB519357; AB519358; AB519359; AB519360; AB519361; AB519362; AB519363; AB519364; AB519365; AB519366; AB519367; AB519368; AB519369; AB519370; AB519371; AB519372; AB519373; AB519374; AB519375; AB519376; AB519377; AB519378; AB519379; AB519380; AB519381; AB519382; AB519383; AB519384; AB519385; AB519386; AB519387; AB519388; AB519389; AB519390; AB519391; AB519392; AB519393; AB519394; AB519395; AB519396; AB519397; AB519398; AB519399; AB519400; AB519401; AB519402; AB519403; AB519404; AB519405; AB519406; AB519407; AB519408; AB519409; AB519410; AB519411; AB519412; AB519413; AB519414; AB519415; AB519416; AB519417; AB519418; AB519419; AB519420; AB519421; AB519422; AB519423; AB519424; AB519425; AB519426; AB519427; AB519428; AB519429; AB519430; AB519431; AB519432; AB519433; AB519434; AB519435; AB519436; AB519437; AB519438; AB519439; AB519440; AB519441; AB519442; AB519443; AB519444; AB519445; AB519446 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cluster Analysis DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics Diet* Electrophoresis, Polyacrylamide Gel Genetic Variation* Metagenome* Molecular Sequence Data Nucleic Acid Denaturation Phylogeny Prevotella / classification*, genetics* RNA, Ribosomal, 16S / genetics Rumen / microbiology* Sequence Analysis, DNA Sheep / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S |
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