Document Detail

Genetic diversity among Paspalum L. species (Poaceae) belonging to the Notata and Linearia groups based on restriction fragment length polymorphism analyses.
MedLine Citation:
PMID:  16247687     Owner:  NLM     Status:  MEDLINE    
A taxonomic study of Paspalum L. was carried out using a genetic diversity approach. Thirty accessions representing twenty one different species from the Notata and Linearia groups of Paspalum were studied using restriction fragment length polymorphism analysis of the amplified ITS ribosomal DNA (rDNA) and from the psbA-trnH of the chloroplast genome (cpDNA). The combined analysis of the internal transcribed spacer (ITS) and the chloroplast spacer region between the psbA and trnH genes identified genetic polymorphisms. A distance analysis of the molecular data generated a dendrogram which showed the relationships of the two informal groups of Paspalum studied here. Although the distribution of species in the dendrogram was found to be roughly in agreement with previous works based on morphological and cytological data, the results obtained reveal the current artificiality in Paspalum taxonomy. Based on molecular data, a new circumscription of the Notata and Linearia groups is proposed here in order to provide a more accurate delimitation of these groups and contribute to the taxonomy of Paspalum. This study, although preliminary, reveals the potential utility of such a molecular approach for clarifying the taxonomy of closely related taxa.
Tiago F Ferreira; Tatiana T Souza-Chies
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetica     Volume:  125     ISSN:  0016-6707     ISO Abbreviation:  Genetica     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-25     Completed Date:  2006-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370740     Medline TA:  Genetica     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Departamento de Genética, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501-970, Porto Alegre-Rio Grande do Sul, Brazil.
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MeSH Terms
DNA, Chloroplast / genetics
DNA, Plant / genetics
DNA, Ribosomal Spacer / genetics
Genetic Variation
Paspalum / classification*,  genetics*
Polymorphism, Restriction Fragment Length
Species Specificity
Reg. No./Substance:
0/DNA, Chloroplast; 0/DNA, Plant; 0/DNA, Ribosomal Spacer

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