Document Detail


Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers.
MedLine Citation:
PMID:  23315808     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Phytophthora capsici is a plant pathogenic oomycete that damages numerous crops worldwide. Consequently, interest in research on the genetic structure of this species has grown in recent decades. However, there is little information about P. capsici in eastern China. We investigated the genetic diversity of P. capsici isolates from three large regions of Anhui Province in eastern China based on ISSR-PCR technology. Thirteen random primers were screened and used to amplify DNA from 51 samples. We obtained 158 reproducible ISSR fragments, of which 90% were polymorphic, revealing a high degree of polymorphism among the isolates. Genetic similarity coefficients among all the isolates ranged from 0.56 to 0.94, with a mean of 0.84 based on the ISSR data, indicating a high level of genetic variation in these P. capsici isolates. Cluster analysis using UPGMA indicated that the Anhui isolates were divided into seven groups according to the DNA fingerprints, although there was no correlation between the ISSR group and geographic origin. Isolates from the same location showed no clustering based on the year of sampling. AMOVA partitioned variability among (13.6%) and within populations (86.4%). The gene flow among populations ranged from 2.804 to 4.937, with a mean of 3.545, indicating highly frequent gene exchange. Genetic distances and genetic differentiation were negatively correlated with geographic distances. These results lead us to suggest that this pathogen has considerable evolutionary potential, which will enable it to adapt to and overcome management strategies over time.
Authors:
P Li; S Cao; Y L Dai; X L Li; D F Xu; M Guo; Y M Pan; Z M Gao
Related Documents :
25033208 - Imad, a genetic screening strategy for dissecting complex interactions between a pathog...
25045658 - Extended genetic diversity of bovine viral diarrhea virus and frequency of genotypes an...
18599088 - The role of genetic biases in shaping the correlations between languages and genes.
24678268 - Evolution of transmission mode in obligate symbionts.
10449138 - No association of alpha-2 macroglobulin gene five-nucleotide deletion with ad in taiwan...
23128498 - Unexplained sudden death, focussing on genetics and family phenotyping.
Publication Detail:
Type:  Journal Article     Date:  2012-12-17
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  11     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2012  
Date Detail:
Created Date:  2013-01-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  4285-96     Citation Subset:  IM    
Affiliation:
School of Plant Protection, Anhui Agricultural University, Hefei, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vect...
Next Document:  Association of the genes for tumor necrosis factor-? and myelin basic protein with delayed encepha...