Document Detail


Genetic dissection of proteolytic and non-proteolytic contributions of MT1-MMP to macrophage invasion.
MedLine Citation:
PMID:  21893028     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
MT1-MMP/MMP-14 is a major invasion-promoting membrane protease expressed in macrophages. In addition to its proteolytic activity that degrades the extracellular matrix, MT1-MMP also boosts ATP production in cells in a manner independent of its proteolytic activity. It remains unclear to what extent the proteolytic and energy-boosting activities of MT1-MMP contribute to macrophage invasion. Recently, we demonstrated that the cytoplasmic tail of MT1-MMP makes use of APBA3/Mint3 to activate HIF-1 and thereby boosts glycolysis for ATP production. Here, we used Apba3(-/-) macrophages to dissect the contribution of the proteolytic and the energy-boosting activities of MT1-MMP. The proteolytic activity of MT1-MMP was not affected by the lack of APBA3 in macrophages. Apba3(-/-) and Mmp14(-/-) macrophages exhibited a 55% reduction of ATP levels compared to wild-type (WT) cells and the rate of motility of the mutant cells was accordingly reduced. In contrast, matrigel invasion by Mmp14(-/-) and Apba3(-/-) macrophages was reduced to 24% and 55.4%, respectively, of the level observed in WT cells. These results represent the first attempt to dissect the contribution of the two invasion-promoting activities of MT1-MMP to macrophage invasion.
Authors:
Toshiro Hara; Kouhei Mimura; Motoharu Seiki; Takeharu Sakamoto
Related Documents :
11022118 - Invertebrate and fish cytokines.
10717188 - Cytokine-induced radiation protection and sensitization.
10357208 - Brefeldin a, but not monensin, completely blocks cd69 expression on mouse lymphocytes: ...
14977558 - Cytokines and fever.
12168568 - Plasminogen activators in inflammation and sepsis.
11935268 - Immunohistochemical analysis of anti-hu-associated paraneoplastic encephalomyelitis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-27
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-9-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
Division of Cancer Cell Research, Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Bam machine: a molecular cooper.
Next Document:  TNF-? increases cardiac fibroblast lysyl oxidase expression through TGF-? and PI3Kinase signaling pa...