Document Detail

Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.).
MedLine Citation:
PMID:  20686747     Owner:  NLM     Status:  MEDLINE    
Powdery mildew caused by Blumeria graminis f. sp. tritici is one of the most important wheat diseases worldwide and breeding for resistance using diversified disease resistance genes is the most promising approach to prevent outbreaks of powdery mildew. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum turgidum var. dicoccoides) accessions collected from Israel, has been transferred into the hexaploid wheat line 3D232 through crossing and backcrossing. Inoculation results with 21 B. graminis f. sp. tritici races indicated that 3D232 is resistant to all of the powdery mildew isolates tested. Genetic analyses of 3D232 using an F(2) segregating population and F(3) families indicated that a single dominant gene, Ml3D232, confers resistance in the host seedling stage. By applying molecular markers and bulked segregant analysis (BSA), we have identified polymorphic simple sequence repeats (SSR), expressed sequence tags (EST) and derived sequence tagged site (STS) markers to determine that the Ml3D232 is located on chromosome 5BL bin 0.59-0.76. Comparative genetic analyses using mapped EST markers and genome sequences of rice and Brachypodium established co-linearity of the Ml3D232 genomic region with a 1.4 Mb genomic region on Brachypodium distachyon chromosome 4, and a 1.2 Mb contig located on the Oryza sativa chromosome 9. Our comparative approach enabled us to develop new EST-STS markers and to delimit the genomic region carrying Ml3D232 to a 0.8 cM segment that is collinear with a 558 kb region on B. distachyon. Eight EST markers, including an NBS-LRR analog, co-segregated with Ml3D232 to provide a target site for fine genetic mapping, chromosome landing and map-based cloning of the powdery mildew resistance gene. This newly developed common wheat germplasm provides broad-spectrum resistance to powdery mildew and a valuable resource for wheat breeding programs.
Hongtao Zhang; Haiying Guan; Jingting Li; Jie Zhu; Chaojie Xie; Yilin Zhou; Xiayu Duan; Tsomin Yang; Qixin Sun; Zhiyong Liu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-05
Journal Detail:
Title:  TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik     Volume:  121     ISSN:  1432-2242     ISO Abbreviation:  Theor. Appl. Genet.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-26     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0145600     Medline TA:  Theor Appl Genet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1613-21     Citation Subset:  IM    
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
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MeSH Terms
Ascomycota / isolation & purification,  physiology*
Base Sequence
Brachypodium / genetics
Chromosome Mapping / methods*
Chromosome Segregation / genetics*
Chromosomes, Plant / genetics
Expressed Sequence Tags
Genes, Plant / genetics*
Immunity, Innate / genetics*
Microsatellite Repeats / genetics
Oryza sativa / genetics
Physical Chromosome Mapping
Plant Diseases / genetics,  immunology*,  microbiology
Sequence Homology, Nucleic Acid
Triticum / genetics*,  immunology,  microbiology

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