Document Detail

Genetic and biochemical characterization of an operon involved in the biosynthesis of 3-deoxy-D-manno-octulosonic acid in Pseudomonas aeruginosa.
MedLine Citation:
PMID:  10220877     Owner:  NLM     Status:  MEDLINE    
A Pseudomonas aeruginosa serotype O5 (PAO1) genomic DNA fragment that was able to complement a temperature-sensitive mutation in the 3-deoxy-D-manno-octulosonic acid (Kdo) 8-P synthase gene (kdsA) of Salmonella enterica serovar typhimurium was cloned. Nucleotide sequence analysis revealed the presence of a potential operon with the gene order pyrG, kdsA, eno. PyrG catalyzes the synthesis of the nucleotide cytidine triphosphate, while Eno catalyzes the formation of phosphoenolpyruvate from phosphoglycerate during glycolysis. Phosphoenolpyruvate is one of the substrates for Kdo-8-P biosynthesis by KdsA and cytidine triphosphate is the nucleotide used to activate Kdo prior to its transfer to lipid A. pyrG and eno are important for many metabolic pathways and it is interesting to find them linked to kdsA. A sigma 70-like promoter was found upstream of pyrG and evidence was provided to show that this promoter was responsible for the initiation of transcription of the genes in this operon. These genes mapped to 28.2-29.9 min on the 75-min PAO1 chromosome, unlinked to other lipopolysaccharide biosynthetic gene clusters.
A G Walsh; L L Burrows; J S Lam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  173     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-07-07     Completed Date:  1999-07-07     Revised Date:  2012-07-13    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Canadian Bacterial Diseases Network, Department of Microbiology, University of Guelph, Ont., Canada.
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MeSH Terms
Aldehyde-Lyases / genetics*,  metabolism
Carbon-Nitrogen Ligases / genetics*,  metabolism
Chromosome Mapping
Chromosomes, Bacterial
DNA-Directed RNA Polymerases
Escherichia coli / genetics
Genetic Complementation Test
Operon / genetics*
Phosphopyruvate Hydratase / genetics*,  metabolism
Physical Chromosome Mapping
Plasmids / genetics
Promoter Regions, Genetic
Pseudomonas aeruginosa / enzymology,  genetics*
Salmonella typhimurium / genetics
Sigma Factor
Sugar Acids / metabolism*
Reg. No./Substance:
0/Sigma Factor; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate; EC aldolase; EC 2.7.7.-/RNA polymerase sigma 70; EC RNA Polymerases; EC 4.1.2.-/Aldehyde-Lyases; EC Hydratase; EC 6.3.-/Carbon-Nitrogen Ligases; EC synthetase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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