| Genetic and biochemical analysis of PadR-padC promoter interactions during the phenolic acid stress response in Bacillus subtilis 168. | |
| | |
MedLine Citation:
|
PMID: 21685295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bacillus subtilis 168 is resistant to phenolic acids by expression of an inducible enzyme, the phenolic acid decarboxylase (PadC), that decarboxylates these acids into less toxic vinyl derivatives. In the phenolic acid stress response (PASR), the repressor of padC, PadR, is inactivated by these acids. Inactivation of PadR is followed by a strong expression of padC. To elucidate the functional interaction between PadR and the padC promoter, we performed (i) footprinting assays to identify the region protected by PadR, (ii) electrophoretic mobility shift assays (EMSAs) with a modified padC promoter protected region to determine the interacting sequences, and (iii) random mutagenesis of padR to identify amino acid residues essential for the function of PadR. We identified an important consensus dyad sequence called IR1-2 (ATGT-8N-ACAT) overlapping a second dyad element (GTGT-8N-ACAT) that we named dIR1-2bis. The entire dIR1-2bis/IR1-2 sequence permits binding of two PadR dimers in EMSAs, which may be observed for bacteria grown under noninduced conditions where the padC promoter is completely repressed. Three groups of modified PadRs giving a PASR phenotype were characterized in vivo. The DNA sequences of certain mutant padR alleles indicate that important residues are all located in the region containing the coiled-coil leucine zipper domain that is involved in dimerization. These substitutions reduce the affinity of PadR binding to the padC promoter. Of particular interest are residue L128, located at the center of the putative coiled-coil leucine zipper domain, and residue E97, which is conserved among all PadRs. |
| | |
Authors:
|
Thi Kim Chi Nguyen; Ngoc Phuong Tran; Jean-François Cavin |
Related Documents
:
|
14449225 - Transient phosphorylation of deoxyribosides and regulation of deoxyribonucleic acid syn... 3145715 - Microbial transformation of the pyrethroid insecticides: permethrin, deltamethrin, fast... 770415 - Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between... 4388385 - Characterization of excess deoxyribonucleic acid synthesized by pneumococci in the pres... 21641455 - An automatic system for acidity determination based on sequential injection titration a... 14628295 - Absolute configuration of the thyroid hormone analog kat-2003 as determined by the 1h n... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-06-17 |
Journal Detail:
|
Title: Journal of bacteriology Volume: 193 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-07-29 Completed Date: 2011-09-27 Revised Date: 2012-02-01 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
|
Languages: eng Pagination: 4180-91 Citation Subset: IM |
Affiliation:
|
Laboratoire GPMA, IFR92, AgroSup Dijon, Université de Bourgogne, 1 Esplanade Erasme, F-21000 Dijon, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
drug effects*,
metabolism*,
physiology Bacterial Proteins / genetics, metabolism* Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial / drug effects* Hydroxybenzoic Acids / pharmacology* Promoter Regions, Genetic / drug effects, physiology Protein Binding Protein Structure, Secondary Stress, Physiological / drug effects* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Hydroxybenzoic Acids; 29656-58-4/phenolic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Refining the binding of the Escherichia coli flagellar master regulator, FlhD4C2, on a base-specific...
Next Document: Crystal structure of Bacillus anthracis phosphoglucosamine mutase, an enzyme in the peptidoglycan bi...