Document Detail

Genetic basis of nutritional requirements in Lactobacillus casei.
MedLine Citation:
PMID:  4215789     Owner:  NLM     Status:  MEDLINE    
In a study of the genetic basis of multiple nutritional requirements in Lactobacillus casei, systematic attempts were made to isolate mutants that can grow in the absence of a specific nutrient required by the parental organism. Such mutants have successfully been isolated with respect to seven of twelve amino acids (aspartic acid, leucine, isoleucine, lysine, methionine, serine, and threonine) and three of four vitamins (pantothenic acid, nicotinic acid, and pyridoxal) tested, after extensive screenings employing various mutagens. Mutants that can grow without tryptophan were not isolated, but those that can grow on anthranilate or indole as well as on tryptophan were obtained at a frequency expected for single-step mutations. Activity of tryptophan synthetase was demonstrated in extracts of these anthranilate-utilizing mutants, but not in the parental strain. These results suggest that the multiple nutritional requirements of L. casei are often, if not always, due to one or a few small lesions such as base substitution mutations rather than large deletions affecting the genes involved in each biosynthetic pathway. The data would also imply that many of the biosynthetic pathways that are not fully functional in L. casei were active at one time and became nonfunctional during evolution of the present species.
T Morishita; T Fukada; M Shirota; T Yura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  120     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Dec 
Date Detail:
Created Date:  1975-02-27     Completed Date:  1975-02-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1078-84     Citation Subset:  IM    
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MeSH Terms
Adenine / analogs & derivatives
Amino Acids / metabolism
Anthranilic Acids / metabolism
Cell-Free System
Culture Media
Indoles / metabolism
Lactobacillus casei / enzymology,  growth & development,  metabolism*
Purines / metabolism
Pyrimidines / metabolism
Tryptophan / metabolism
Tryptophan Synthase / metabolism
Ultraviolet Rays
Vitamins / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Anthranilic Acids; 0/Culture Media; 0/Indoles; 0/Mutagens; 0/Nitrosoguanidines; 0/Purines; 0/Pyrimidines; 0/Vitamins; 73-22-3/Tryptophan; 73-24-5/Adenine; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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