Document Detail


Genetic analysis of synaptotagmin C2 domain specificity in regulating spontaneous and evoked neurotransmitter release.
MedLine Citation:
PMID:  23283333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Synaptic vesicle fusion mediates communication between neurons and is triggered by rapid influx of Ca(2+). The Ca(2+)-triggering step for fusion is regulated by the synaptic vesicle transmembrane protein Synaptotagmin 1 (Syt1). Syt1 contains two cytoplasmic C2 domains, termed C2A and C2B, which coordinate Ca(2+) binding. Although C2A and C2B share similar topology, binding of Ca(2+) ions to the C2B domain has been suggested as the only critical trigger for evoked vesicle release. If and how C2A domain function is coordinated with C2B remain unclear. In this study, we generated a panel of Syt1 chimeric constructs in Drosophila to delineate the unique and shared functions of each C2 domain in regulation of synaptic vesicle fusion. Expression of Syt 1 transgenes containing only individual C2 domains, or dual C2A-C2A or C2B-C2B chimeras, failed to restore Syt1 function in a syt1(-/-) null mutant background, indicating both C2A and C2B are specifically required to support fast synchronous release. Mutations that disrupted Ca(2+) binding to both C2 domains failed to rescue evoked release, but supported synaptic vesicle docking and endocytosis, indicating that these functions of Syt1 are Ca(2+)-independent. The dual C2 domain Ca(2+)-binding mutant also enhanced spontaneous fusion while dramatically increasing evoked release when coexpressed with native Syt1. Together, these data indicate that synaptic transmission can be regulated by Syt1 multimerization and that both C2 domains of Syt1 are uniquely required for modulating Ca(2+)-independent spontaneous fusion and Ca(2+)-dependent synchronous release.
Authors:
Jihye Lee; Zhuo Guan; Yulia Akbergenova; J Troy Littleton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  33     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-03-12     Revised Date:  2013-07-18    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-200     Citation Subset:  IM    
Affiliation:
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. jihyelee@mit.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Drosophila
Mutation
Neurons / metabolism*
Synaptic Transmission / genetics*
Synaptic Vesicles / genetics*,  metabolism
Synaptotagmin I / genetics*,  metabolism
Transgenes
Grant Support
ID/Acronym/Agency:
NS40296/NS/NINDS NIH HHS; R01 NS040296/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Synaptotagmin I; 7440-70-2/Calcium
Comments/Corrections

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