Document Detail


Genetic analysis of simian immunodeficiency virus expressed in milk and selectively transmitted through breastfeeding.
MedLine Citation:
PMID:  16571789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To develop effective intervention strategies that prevent breast milk transmission of human immunodeficiency virus (HIV), we must understand the specific viral properties and mechanisms responsible for infant infection. We have used lactating rhesus macaques infected with a pathogenic simian immunodeficiency virus (SIV) stock to analyze the viral genotypes expressed in plasma and milk throughout the disease course and to identify those variants ultimately transmitted to infants through breastfeeding. In these studies we observed mother-to-infant transmission of SIV/Delta(B670) by eight females during the chronic phase of disease, and we analyzed by heteroduplex tracking assays and sequence analysis the distribution and fluctuations in viral genotypes expressed. Each female expressed multiple V1 envelope genotypes in milk near the time of transmission, while a single genotype was found in each of the infants. Variants transmitted to infants were not expressed throughout the maternal disease course but were only detected near the time of transmission. The emergence of the transmitted genotype in the dam typically occurred in plasma before milk and was coincident with increased milk viral loads. Transmitted genotypes tended to be longer and more glycosylated and had a less negative charge over the V1 region compared to viral genotypes expressed in milk but not transmitted. These observations demonstrate that specific viral genotypes are selectively transmitted to infants through breastfeeding and support the hypothesis that transmission occurs as genotypes adapt for efficient expression in milk.
Authors:
Jenna Rychert; Nedra Lacour; Angela Martin Amedee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  80     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-30     Completed Date:  2006-04-24     Revised Date:  2014-01-17    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3721-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Breast Feeding*
Female
Genotype
Infectious Disease Transmission, Vertical*
Macaca mulatta
Milk / virology*
Molecular Sequence Data
Simian Acquired Immunodeficiency Syndrome / transmission*
Simian immunodeficiency virus / classification,  genetics*
Viral Load
Grant Support
ID/Acronym/Agency:
R01 DE012916/DE/NIDCR NIH HHS; R01 DE012916-01/DE/NIDCR NIH HHS
Comments/Corrections

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