Document Detail


Genetic analysis of daily physical activity using a mouse chromosome substitution strain.
MedLine Citation:
PMID:  19567786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is considerable evidence for a genetic basis underlying individual differences in spontaneous physical activity in humans and animals. Previous publications indicate that the physical activity level and pattern vary among inbred strains of mice and identified a genomic region on chromosome 13 as quantitative trait loci (QTL) for physical activity. To confirm and further characterize the role of chromosome 13 in regulating daily physical activity level and pattern, we conducted a comprehensive phenotypic study in the chromosome 13 substitution strain (CSS-13) in which the individual chromosome 13 from the A/J strain was substituted into an otherwise complete C57BL/6J (B6) genome. The B6 and A/J parental strains exhibited pronounced differences in daily physical activity, sleep-wake structure, circadian period and body weight. Here we report that a single A/J chromosome 13 in the context of a B6 genetic background conferred a profound reduction in both total cage activity and wheel-running activity under a 14:10-h light-dark cycle, as well as in constant darkness, compared with B6 controls. Additionally, CSS-13 mice differed from B6 controls in the diurnal distribution of activity and the day-to-day variability in activity onset. We further performed a linkage analysis and mapped a significant QTL on chromosome 13 regulating the daily wheel running activity level in mice. Taken together, our findings indicate a QTL on chromosome 13 with dramatic and specific effects on daily voluntary physical activity, but not on circadian period, sleep, or other aspects of activity that are different between B6 and A/J strains.
Authors:
He S Yang; Martha H Vitaterna; Aaron D Laposky; Kazuhiro Shimomura; Fred W Turek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-06-30
Journal Detail:
Title:  Physiological genomics     Volume:  39     ISSN:  1531-2267     ISO Abbreviation:  Physiol. Genomics     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-10     Completed Date:  2010-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815683     Medline TA:  Physiol Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  47-55     Citation Subset:  IM    
Affiliation:
Center for Sleep and Circadian Biology, Northwestern University, Evanston, Illinois 60208-3520, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight
Chromosomes, Mammalian / genetics*
Crosses, Genetic
Female
Infrared Rays
Male
Mice
Mice, Inbred Strains
Motor Activity / genetics*
Quantitative Trait Loci / genetics
Sleep / genetics,  physiology
Wakefulness / genetics,  physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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