| Genetic analysis of basophil function in vivo. | |
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MedLine Citation:
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PMID: 21552267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Contributions by basophils to allergic and helminth immunity remain incompletely defined. Using sensitive interleukin 4 (Il4) reporter alleles, we demonstrate here that basophil IL-4 production occurs by a CD4(+) T cell-dependent process restricted to the peripheral tissues affected. We genetically marked and achieved specific deletion of basophils and found that basophils did not mediate T helper type 2 (T(H)2) priming in vivo. Two-photon imaging confirmed that basophils did not interact with antigen-specific T cells in lymph nodes but engaged in prolonged serial interactions with T cells in lung tissues. Although targeted deletion of IL-4 and IL-13 in either CD4(+) T cells or basophils had a minimal effect on worm clearance, deletion from both lineages demonstrated a nonredundant role for basophil cytokines in primary helminth immunity. |
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Authors:
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Brandon M Sullivan; Hong-Erh Liang; Jennifer K Bando; Davina Wu; Laurence E Cheng; James K McKerrow; Christopher D C Allen; Richard M Locksley |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-05-08 |
Journal Detail:
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Title: Nature immunology Volume: 12 ISSN: 1529-2916 ISO Abbreviation: Nat. Immunol. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-18 Completed Date: 2011-07-22 Revised Date: 2012-02-06 |
Medline Journal Info:
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Nlm Unique ID: 100941354 Medline TA: Nat Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 527-35 Citation Subset: IM |
Affiliation:
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Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basophils / immunology*, metabolism CD4-Positive T-Lymphocytes / immunology, metabolism Female Flow Cytometry Green Fluorescent Proteins / genetics, metabolism Helminthiasis, Animal / immunology, metabolism, parasitology Host-Parasite Interactions / immunology Humans Interleukin-13 / genetics, immunology, metabolism Interleukin-4 / genetics, immunology*, metabolism Liver / immunology, metabolism, parasitology Lung / immunology*, metabolism, parasitology Lung Diseases, Parasitic / immunology, metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Microscopy, Fluorescence, Multiphoton Nippostrongylus / immunology, physiology Schistosoma mansoni / immunology, physiology Strongylida Infections / immunology*, metabolism, parasitology |
| Grant Support | |
ID/Acronym/Agency:
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AI026918/AI/NIAID NIH HHS; AI077439/AI/NIAID NIH HHS; R37 AI026918-24/AI/NIAID NIH HHS; U19 AI077439-05/AI/NIAID NIH HHS; //Howard Hughes Medical Institute; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-13; 147336-22-9/Green Fluorescent Proteins; 207137-56-2/Interleukin-4 |
| Comments/Corrections | |
Erratum In:
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Nat Immunol. 2011 Jun;12(6):3 p following 535 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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