| Genetic alterations in metastatic renal cell carcinoma detected by comparative genomic hybridization: correlation with clinical and histological data. | |
| | |
MedLine Citation:
|
PMID: 11029490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In order to optimize the management of patients with renal cell carcinoma (RCC) it is important to define the genetic risk for metastatic disease. In this study we performed comparative genomic hybridization (CGH) on metastatic tumors aiming at the identification of genetic alterations associated with metastatic disease. We analyzed 46 renal tumors along with their metastases, and 15 non-metastatic renal tumors. Tumors were classified pathologically according to the Heidelberg classification of RCC, and staged according to the TNM-system. Standard CGH was performed using microdissected archival tissues and DOP-PCR. The average numbers of chromosomal aberrations per tumor were 3.0, 2.1 and 3.9 in patients without metastasis, in patients who developed metastases after a two-year latency period (late onset of metastatic disease) and in patients who developed metastases within two years after therapy of the primary tumor (early onset of metastatic disease). CGH revealed chromosomal aberrations in 91% of primary metastatic tumors. Deletions or losses of chromosomes 9 (26% vs 6%), 10 (21% vs 6%) and 18 (23% vs 0) and 17 (28% vs 7%) occurred more often in metastatic tumors than in non-metastatic tumors. Furthermore, these aberrations were more common in patients with early metastases. CGH analysis of 40 pairs of primary RCCs and their corresponding metastasis revealed similar aberrations in 70% of cases. In 30%, however, metastases showed additional chromosomal aberrations not detected in the corresponding primary tumors. In conclusion, we identified genetic alterations associated with metastatic disease in RCC which could be useful for predicting prognosis. Genetic changes leading to metastases occurred early in tumorigenesis of metastatic tumors. |
| | |
Authors:
|
K Junker; P Moravek; M Podhola; G Weirich; W Hindermann; V Janitzky; J Schubert |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: International journal of oncology Volume: 17 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2000 Nov |
Date Detail:
|
Created Date: 2000-12-05 Completed Date: 2000-12-05 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: GREECE |
Other Details:
|
Languages: eng Pagination: 903-8 Citation Subset: IM |
Affiliation:
|
Department of Urology, D-07743 Jena, Germany. kerstin.junker@med.uni-jena.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carcinoma, Renal Cell
/
genetics*,
pathology Chromosome Aberrations* Chromosome Deletion Chromosomes, Human / genetics, ultrastructure DNA Mutational Analysis DNA, Neoplasm / genetics* Disease Progression Humans Kidney Neoplasms / genetics*, pathology Neoplasm Metastasis Nucleic Acid Hybridization* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Neoplasm |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Rb family of cell cycle regulatory factors: clinical implications.
Next Document: Expression in hepatomas and chromosomal localization of rat protein phosphatase 5 gene.