| Genetic alterations in DNA diploid, aneuploid and multiploid colorectal carcinomas identified by the crypt isolation technique. | |
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MedLine Citation:
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PMID: 11058879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Loss of heterozygosity (LOH) and microsatellite instability (MSI) commonly occur in colorectal carcinomas. However, the role of these genetic alterations in determining DNA ploidy status of tumors (diploid, aneuploid and multiploid) remains unclear. In the present study, we attempted to clarify the relationship between genetic alterations and DNA ploidy status. Crypt isolation coupled with DNA cytometric sorting and polymerase chain reaction assay (17 microsatellite markers) were used to study allelic losses and MSI in 59 colorectal carcinomas (diploid, 15; aneuploid, 10 and multiploid, 34). Of the 15 diploid carcinomas, 6 exhibited MSI in which allelic losses were rarely found. The other 9 diploid tumors mostly exhibited allelic losses, but none displayed MSI status. Whereas allelic losses frequently occurred in the aneuploid carcinomas and the aneuploid populations of multiploid carcinomas, they were rarely detected in the diploid populations of multiploid carcinomas. MSI status was not observed in aneuploid carcinomas nor in either population of multiploid carcinomas. Although multiploid carcinomas genetically resemble aneuploid carcinomas in the expression of the severe LOH phenotype, the genetic alterations seen in the diploid populations of multiploid carcinomas may differ from those of diploid carcinomas. Furthermore, all diploid, aneuploid and both the diploid and aneuploid fractions of the multiploid tumors that were non-MSI exhibited a high rate of LOH, suggesting that LOH is independent of the tumor's ploidy status. |
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Authors:
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T Sugai; W Habano; S Nakamura; H Sato; N Uesugi; H Takahashi; Y Jiao; T Yoshida; C Itoh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 88 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-08 Completed Date: 2000-12-08 Revised Date: 2007-07-24 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 614-9 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
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Division of Pathology, Central Clinical Laboratory, Iwate Medical University, Morioka, Japan. tsugai@cocoa.ocn.ne.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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genetics,
pathology Adenocarcinoma, Mucinous / genetics, pathology Adult Aged Aged, 80 and over Aneuploidy Chromosomes, Human, Pair 1 Chromosomes, Human, Pair 17 Chromosomes, Human, Pair 5 Chromosomes, Human, Pair 8 Colorectal Neoplasms / genetics*, pathology* DNA, Neoplasm / genetics Diploidy Female Genetic Markers Humans Loss of Heterozygosity* Male Microsatellite Repeats Middle Aged Ploidies* Polyploidy |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Genetic Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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