Document Detail

Genetic alterations in DNA diploid, aneuploid and multiploid colorectal carcinomas identified by the crypt isolation technique.
MedLine Citation:
PMID:  11058879     Owner:  NLM     Status:  MEDLINE    
Loss of heterozygosity (LOH) and microsatellite instability (MSI) commonly occur in colorectal carcinomas. However, the role of these genetic alterations in determining DNA ploidy status of tumors (diploid, aneuploid and multiploid) remains unclear. In the present study, we attempted to clarify the relationship between genetic alterations and DNA ploidy status. Crypt isolation coupled with DNA cytometric sorting and polymerase chain reaction assay (17 microsatellite markers) were used to study allelic losses and MSI in 59 colorectal carcinomas (diploid, 15; aneuploid, 10 and multiploid, 34). Of the 15 diploid carcinomas, 6 exhibited MSI in which allelic losses were rarely found. The other 9 diploid tumors mostly exhibited allelic losses, but none displayed MSI status. Whereas allelic losses frequently occurred in the aneuploid carcinomas and the aneuploid populations of multiploid carcinomas, they were rarely detected in the diploid populations of multiploid carcinomas. MSI status was not observed in aneuploid carcinomas nor in either population of multiploid carcinomas. Although multiploid carcinomas genetically resemble aneuploid carcinomas in the expression of the severe LOH phenotype, the genetic alterations seen in the diploid populations of multiploid carcinomas may differ from those of diploid carcinomas. Furthermore, all diploid, aneuploid and both the diploid and aneuploid fractions of the multiploid tumors that were non-MSI exhibited a high rate of LOH, suggesting that LOH is independent of the tumor's ploidy status.
T Sugai; W Habano; S Nakamura; H Sato; N Uesugi; H Takahashi; Y Jiao; T Yoshida; C Itoh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  88     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-08     Completed Date:  2000-12-08     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  614-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Division of Pathology, Central Clinical Laboratory, Iwate Medical University, Morioka, Japan.
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MeSH Terms
Adenocarcinoma / genetics,  pathology
Adenocarcinoma, Mucinous / genetics,  pathology
Aged, 80 and over
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 8
Colorectal Neoplasms / genetics*,  pathology*
DNA, Neoplasm / genetics
Genetic Markers
Loss of Heterozygosity*
Microsatellite Repeats
Middle Aged
Reg. No./Substance:
0/DNA, Neoplasm; 0/Genetic Markers

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