Document Detail


Genetic alterations and chemosensitivity profile in newly established human renal collecting duct carcinoma cell lines.
MedLine Citation:
PMID:  19154479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine the genetic alterations and chemosensitivity profile of collecting duct carcinoma (CDC) of the kidney, as it is a rare, highly aggressive malignant tumour with frequent distant metastases. MATERIALS AND METHODS: We first established and characterized two human CDC cell lines designated AP3 and AP8, respectively. The CDC cell lines were assessed using microarray-based comparative genomic hybridization and chemosensitivity testing. RESULTS: The CDC cells grew in vitro as an adherent monolayer with epithelial morphology, but had different growth rates. The cell lines had the characteristic immunophenotype of CDC (high molecular weight cytokeratin-+ve/cytokeratin 7-+ve/vimentin-+ve). Both cell lines shared copy number gains in chromosomes 20 and X. The loci showing a copy number gain were SOX22 at 20p tel, topoisomerse I (TOP1) at 20q12-q13.1, TPD52L2 at 20q tel, 20QTEL14 at 20q tel, KAL at Xp22.3, STS 5' at Xp22.3, OCRL1 at Xq25, AR3'at Xq11-q12, and XIST at Xq13.2, respectively. Immunoblot analysis confirmed that the AP3 and AP8 cell lines showed moderate and high levels of TOP1 expression, respectively. By chemosensitivity testing, the AP8 cells were most sensitive to topoisomerase I and II inhibitors such as topotecan, epirubicin and doxorubicin, but the AP3 cells did not. The chemosensitivity to these drugs was paralleled by cell death via apoptosis. CONCLUSION: The results suggest that TOP1 might be one of the molecular targets in AP8 CDC cells. Thus, these novel CDC cell lines will be useful for discovering therapeutic targets and developing effective anticancer drugs against CDC.
Authors:
Zheng-Sheng Wu; Ju-Han Lee; Jung-Ah Kwon; Seo-Hee Kim; Sun-Hee Han; Jung-Suk An; Ji-Hye Lee; Eung-Seok Lee; Heum-Rye Park; Young-Sik Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-09
Journal Detail:
Title:  BJU international     Volume:  103     ISSN:  1464-410X     ISO Abbreviation:  BJU Int.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-23     Completed Date:  2009-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100886721     Medline TA:  BJU Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1721-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, Korea University Ansan Hospital, Ansan, Korea.
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MeSH Terms
Descriptor/Qualifier:
Aged
Blotting, Western
Carcinoma, Renal Cell / drug therapy,  genetics*
Cell Line, Tumor
Comparative Genomic Hybridization
DNA Topoisomerases, Type I / genetics
Female
Humans
Immunohistochemistry
Kidney Neoplasms / drug therapy,  genetics*
Kidney Tubules, Collecting*
Male
Middle Aged
Chemical
Reg. No./Substance:
EC 5.99.1.2/DNA Topoisomerases, Type I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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