| Genetic activity of actinomycin D in Saccharomyces cerevisiae but not in Escherichia coli. | |
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MedLine Citation:
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PMID: 7022197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potential of actinomycin D for induction of forward mutation (ADE-), reversion (TRP+), gene conversion, and mitotic recombination, was examined using haploid and diploid strains of yeast Saccharomyces cerevisiae. No increase in forward or reverse mutations or gene conversion was detected, but actinomycin D induced up to 13-fold increases in mitotic recombinants and a 2-fold increase in numbers of aberrant colonies, in a non-selective assay for genetic activity. Actinomycin D was non-mutagenic in a fluctuation test using Escherichia coli strain WP2 UvrA-. This furnishes an example of a mutagen which is negative in bacteria but has genetic activity in yeast, emphasizing the need for using a battery of microbial tests for determining the genetic activity of any given chemical. |
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Authors:
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E R Nestmann; A Nasim; R H Haynes; D J Kowbel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 89 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1981 Jul |
Date Detail:
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Created Date: 1981-10-25 Completed Date: 1981-10-25 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 229-36 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dactinomycin
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pharmacology* Escherichia coli / genetics Mutagenicity Tests Mutagens* Saccharomyces cerevisiae / genetics |
| Chemical | |
Reg. No./Substance:
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0/Mutagens; 50-76-0/Dactinomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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