|Genetic Variants of Orientia tsutsugamushi in Domestic Rodents, Northern China.|
|Jump to Full Text|
|PMID: 23764295 Owner: NLM Status: In-Data-Review|
|We screened Orientia tsutsugamushi from 385 domestic rodents and 19 humans with scrub typhus in rural Tai'an District, Shandong Province, a new scrub typhus epidemic area in northern China. Sequence analysis identified 7 genotypes in the rodents, of which 2 were also identified in the humans.|
|Meng Zhang; Zhong-Tang Zhao; Xian-Jun Wang; Zhong Li; Lei Ding; Shu-Jun Ding; Hui-Li Yang|
Related Documents :
|17248975 - Identification of an r-locus region that controls the tissue specificity of anthocyanin...
15136915 - Transformation of spirulina platensis strain c1 (arthrospira sp. pcc9438) with tn5 tran...
23394135 - Genetic structure in cultivated grapevines is linked to geography and human selection.
19053395 - Molecular level lignin patterns of genetically modified bt-maize mon88017 and three con...
23188175 - The red queen in the corn: agricultural weeds as models of rapid adaptive evolution.
19132115 - The fission yeast model for the lysosomal storage disorder batten disease predicts dise...
|Type: Journal Article|
|Title: Emerging infectious diseases Volume: 19 ISSN: 1080-6059 ISO Abbreviation: Emerging Infect. Dis. Publication Date: 2013 Jul|
|Created Date: 2013-06-14 Completed Date: - Revised Date: -|
Medline Journal Info:
|Nlm Unique ID: 9508155 Medline TA: Emerg Infect Dis Country: United States|
|Languages: eng Pagination: 1135-7 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Journal ID (nlm-ta): Emerg Infect Dis
Journal ID (iso-abbrev): Emerging Infect. Dis
Journal ID (publisher-id): EID
Publisher: Centers for Disease Control and Prevention
Print publication date: Month: 7 Year: 2013
Volume: 19 Issue: 7
First Page: 1135 Last Page: 1137
PubMed Id: 23764295
Publisher Id: 12-0984
|Genetic Variants of Orientia tsutsugamushi in Domestic Rodents, Northern China Alternate Title:Orientia tsutsugamushi in Domestic Rodents, China|
|Shandong University, Jinan, People’s Republic of China (M. Zhang, Z.-T. Zhao, L. Ding);
|Shandong Center for Disease Control and Prevention, Jinan (X.-J. Wang, Z. Li, S.-J. Ding);
|Tai’an Center for Disease Control and Prevention, Tai’an, China (H.-L. Yang)
|Correspondence: Address for correspondence: Zhong-Tang Zhao, Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, No. 44 Wenhuaxi Rd, Jinan 250012, China; email: email@example.com
Orientia tsutsugamushi, the causative agent of scrub typhus, is widely prevalent in the Asia–Pacific region and causes an estimated 1 million cases per year (1). It is characterized by dramatic genetic diversity (2). In the 1960s, complement fixation initially identified O. tsutsugamushi as Karp, Gilliam, and Kato types (3), which are widely used as tested antigens in serologic assays. Since then, >20 antigenic variants of O. tsutsugamushi have been identified by immunologic and molecular methods (2). The 56-kDa type-specific antigen (TSA), which is one of the major immunogens of the agent and is associated with pathogenesis, has been commonly used for type designation (4–6).
Scrub typhus is a traditional tropical rickettsiosis. However, since 1986, it has emerged and spread rapidly in temperate zones; the epidemic season is mainly in autumn and winter (7,8). In the late 1990s, the district of Tai’an, west of Shandong Province, northern China (116°20′–117°59′E, 35°38′–36°28′N), became a new epidemic area of autumn–winter type scrub typhus (9); however, few epidemiologic and molecular investigations of the transmission cycle of the disease have been conducted in this area. We therefore investigated the prevalence of O. tsutsugamushi infection among rural domestic rodents in the newly developed epidemic area, evaluated the genotypic diversity according to the variations in the 56-kDa TSA gene, and explored the genetic relationship between strains circulating among domestic rodents and those infecting humans.
From September 2010 through March 2012, rodents were trapped in rural residences from 2 county-level divisions (Xintai and Daiyue) in Tai’an. After each rodent was numbered and species identified, the spleen was dissected for DNA extraction.
During 2010–2011, scrub typhus patients from Xintai and Daiyue were recruited from 7 hospitals in Tai’an. Before the patients received antimicrobial drugs, whole blood samples were collected and anticoagulated with EDTA-Na2. Eschar specimens, if available, were collected after spontaneous desquamation.
DNA was extracted from each specimen by using a TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). Considering the higher sensitivity and specificity, seminested PCR was used to amplify the coding sequences spanning variable domain I–VD III of the 56-kDa TSA with previously described primers A (5′-TTTCGAACGTGTCTTTAAGC-3′), B (5′-ACAGATGCACTATTAGGCAA-3′), and E (5′-GTTGGAGGAATGATTACTGG-3′) (10), which yielded fragments of ≈733 nt. To avoid contamination, PCR preparation was performed in an area separate from DNA extraction, and negative controls were included in each step. Nucleotide sequences of O. tsutsugamushi determined in this study were deposited in GenBank under accession nos. JX202566–JX202568, JX202573, JX202578, JX202579, and JX202580–JX202589. Theses sequences were aligned with 38 reference sequences of the O. tsutsugamushi 56k-Da TSA gene retrieved from GenBank by using MEGA software with the ClustalW algorithm (11). The phylogenetic tree was constructed by using MEGA software with the minimum-evolution method and the Kimura 2-parameter model, with bootstrapping for 1,000 replications. A percentage nucleotide identity matrix was calculated by using MegAlign (Lasergene; DNASTAR Inc., Madison, WI, USA).
The species of the 385 captured rodents were 244 Rattus norvegicus, 139 Mus musculus, and 2 R. rattus. O. tsutsugamushi DNA was detected by PCR in 10 (2.6%) rodents captured in October, November, December, and March; 6 (4.3%) rodents were M. musculus and 4 (1.6%) were R. norvegicus. Rates of O. tsutsugamushi positivity in domestic rodents captured in spring, summer, autumn, and winter were 5.3%, 0, 3.1%, and 1.2%, respectively (Table). No significant difference was observed in the rate of O. tsutsugamushi positivity determined by PCR among the 3 species of rodents (p = 0.278) or among seasons (p = 0.274).
Among the 10 sequences obtained from rodents, we identified 3 genogroups (Kawasaki-related, Fuji-related, and novel genogroup) involving 7 genotypes with >1 nt difference. The genotypes showed 69.7%–99.9% identity with each other. M. musculus and R. norvegicus rodents in Tai’an could serve as reservoirs of 5 and 3 genotypes, respectively. Genotypes KWS1–4 clustered with the Kawasaki genotype (96%–96.5% identity). Genotype FJS was closely related to the Fuji genotype (96.5% identity), which was originally isolated from Leptotrombidium fuji mites in Japan (12). Genotypes SDM1 and SDM2 clustered in an independent clade, which had 70.4%–77.9% identity with reference strains (Figure).
Whole blood or eschar specimens were obtained from 19 human patients, among whom 6 (31.6%) were infected with O. tsutsugamushi according to seminested PCR. Two genotypes (KWS1 and KWS2), which were identified in domestic rodents and belonged to Kawasaki-related genogroups, were also found in the patients (Figure).
Our study demonstrated the prevalence of 7 genotypes of O. tsutsugamushi in the new epidemic area in Tai’an, northern China. Genetic diversity of O. tsutsugamushi was observed within rodent species. Given that KWS1 and KWS2 identified in domestic rodents could cause human infection, prevalence of O. tsutsugamushi infection in domestic rodents might be a public health concern. Natural infection with the KWS2 genotype of O. tsutsugamushi in rodents was found during March in Tai’an. Periodic molecular surveillance of O. tsutsugamushi in local areas might help predict changes in the epidemic features of scrub typhus. Physicians should not arbitrarily exclude a diagnosis of scrub typhus for patients with fever of unknown origin during nonepidemic seasons.
The Kawasaki and Fuji strains of O. tsutsugamushi were considered to be of low virulence (12,13). Cross-reactivities of the Kawasaki strain with 1–18 anti-Gilliam monoclonal antibodies and of the Fuji strain with 5D-3 anti-Kato monoclonal antibodies were observed (12). The 56-kDa TSA is responsible for adhesion to and invasion of the host cells, and its variable domains might serve as epitopes (5,14). Although the identity of the KWS1–4 and FJS genotypes and their prototypes was >96%, changes in virulence and antigenicity might occur because of minor variation. Among the scrub typhus patients from Xintai and Daiyue, Kawasaki-related genotypes were predominant. Antigenic types of these genotypes remain to be determined. Those that are antigenically distinguished from the commonly used antigens should be included in the antigen panel of serologic assays in the local area, which would prompt the diagnosis and prevention of scrub typhus for local residents and travelers.
Identity of SDM1 and SDM2 with the reference strains was <77.9%, and they formed a distinct cluster, indicating that they might represent novel genotypes. Infection of scrub typhus patients with SDM1, SDM2, and Fuji-related genotypes has not been reported. Investigation of host ranges, invasiveness, and virulence of newly defined genotypes of O. tsutsugamushi in local areas will help determine their pathogenic potential for humans.
Expansion of genetic diversity of O. tsutsugamushi was expected to occur in maintenance hosts (15). We are currently screening O. tsutsugamushi in chigger mites collected from the captured rodents to evaluate the role of domestic rodents in the transmission of scrub typhus to humans. Assiduous surveillance of genetic variations of O. tsutsugamushi in hosts and identification of their pathogenic potentials is essential for the improvement of diagnostic capacity, vaccine development, and assessment of epidemiologic role.
Suggested citation for this article: Zhang M, Zhao Z-T, Wang X-J, Li Z, Ding L, Ding S-J, et al. Genetic variants of Orientia tsutsugamushi in domestic rodents, northern China. Emerg Infect Dis [Internet]. 2013 Jul [date cited].
We thank Ai-Hua Zhang, Jia-Qin Xue, Xiang-Peng Meng, and Chen Chen for their contributions to sample collection.
This work was supported by grants from the National Natural Science Foundation of China (nos. 81273133, 30972515).
Ms Zhang is a PhD candidate in the Department of Epidemiology and Health Statistics, School of Public Health, Shandong University. Her primary research interests focus on the molecular epidemiology of O. tsutsugamushi.
|1. .||WattG, ParolaP. Scrub typhus and tropical rickettsioses.Curr Opin Infect Dis. Year: 2003;16:429–36 . 10.1097/00001432-200310000-0000914501995|
|2. .||KellyDJ, FuerstPA, ChingWM, RichardsAL. Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientia tsutsugamushi.Clin Infect Dis. Year: 2009;48(suppl3):S203–3010.1086/59657619220144|
|3. .||ShishidoA. Identification and serological classification of the causative agent of scrub typhus in Japan.Jpn J Med Sci Biol. Year: 1962;15:308–21|
|4. .||HansonB. Identification and partial characterization of Rickettsia tsutsugamushi major protein immunogens.Infect Immun. Year: 1985;50:603–9 .2415453|
|5. .||UrakamiH, TsuruharaT, TamuraA. Penetration of Rickettsia tsutsugamushi into cultured mouse fibroblasts (L cells): an electron microscopic observation.Microbiol Immunol. Year: 1983;27:251–63 .6410159|
|6. .||OhashiN, NashimotoH, IkedaH, TamuraA. Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes encoding TSA homologues from four antigenic variants.J Biol Chem. Year: 1992;267:12728–35 .1618776|
|7. .||KimYS, YunHJ, ShimSK, KooSH, KimSY, KimS. A comparative trial of a single dose of azithromycin versus doxycycline for the treatment of mild scrub typhus.Clin Infect Dis. Year: 2004;39:1329–3510.1086/42500815494910|
|8. .||LiuYX, FengD, SuoJJ, XingYB, LiuG, LiuLH, et al. Clinical characteristics of the autumn–winter type scrub typhus cases in south of Shandong Province, northern China.BMC Infect Dis. Year: 2009;9:8210.1186/1471-2334-9-8219493361|
|9. .||XueJQ, YangHL, ChenY. A study of Orientia tsutsugamushi infection in the area surrounding Mount Tai and gene sequence analysis.Journal of Pathogen Biology.Year: 2010;5:512–6|
|10. .||EnatsuT, UrakamiH, TamuraA. Phylogenetic analysis of Orientia tsutsugamushi strains based on the sequence homologies of 56-kDa type-specific antigen genes.FEMS Microbiol Lett. Year: 1999;180:163–910.1111/j.1574-6968.1999.tb08791.x10556707|
|11. .||TamuraK, PetersonD, PetersonN, StecherG, NeiM, KumarS. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Mol Biol Evol. Year: 2011;28:2731–910.1093/molbev/msr12121546353|
|12. .||TamuraA, MakisakaY, KadosakaT, EnatsuT, OkuboK, KoyamaS, et al. Isolation of Orientia tsutsugamushi from Leptotrombidium fuji and its characterization.Microbiol Immunol. Year: 2000;44:201–4 .10789509|
|13. .||NaganoI, KasuyaS, NodaN, YamashitaT. Virulence in mice of Orientia tsutsugamushi isolated from patients in a new endemic area in Japan.Microbiol Immunol. Year: 1996;40:743–7 .8981347|
|14. .||SeongSY, KimMK, LeeSM, OdgerelZ, ChoiMS, HanTH, et al. Neutralization epitopes on the antigenic domain II of the Orientia tsutsugamushi 56-kDa protein revealed by monoclonal antibodies.Vaccine. Year: 2000;19:2–910.1016/S0264-410X(00)00167-510924780|
|15. .||DuongV, BlassdellK, MayTT, SreyrathL, GavotteL, MorandS, et al. Diversity of Orientia tsutsugamushi clinical isolates in Cambodia reveals active selection and recombination process. Infect Genet Evol. Year: 2010 Sep 18 [Epub ahead of print].|
|Rodent species||Season, no. positive/no. tested (%)
|Mus musculus||2/18 (11.1)||0/21 (0)||3/60 (5.0)||1/40 (2.5)||6/139 (4.3)|
|Rattus norvegicus||1/39 (2.6)||0/31 (0)||3/131 (2.3)||0/43 (0)||4/244 (1.6)|
|*Determined by PCR.|
Keywords: Keywords: scrub typhus, Orientia tsutsugamushi, genotype, phylogeny, parasites, China, rodents, humans.
Previous Document: Influence of humans on evolution and mobilization of environmental antibiotic resistome.
Next Document: Focal corticothalamic sources during generalized absence seizures: A MEG study.