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Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle.
MedLine Citation:
PMID:  25348299     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Human papillomaviruses are the causative agents of several cancers, but only a minority of HPV infections progress to malignancy. In order to better understand HPV biology during the normal, differentiation-dependent life cycle, a cell culture model that maintains the complete episomal genome and permits host cell differentiation is critical. Furthermore, the use of cloned DNA as a starting material is important to facilitate genetic analyses. In this chapter, procedures for isolating human keratinocytes, establishing cell lines maintaining HPV16 genomes, and inducing cellular differentiation, which permits analysis of both early and late stages in the viral life cycle, are described.
Authors:
Jason M Bodily
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1249     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2015  
Date Detail:
Created Date:  2014-10-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  81-91     Citation Subset:  IM    
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