| A genetic mechanism for emergence of races in Fusarium oxysporum f. sp. lycopersici: inactivation of avirulence gene AVR1 by transposon insertion. | |
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MedLine Citation:
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PMID: 22952887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Compatible/incompatible interactions between the tomato wilt fungus Fusarium oxysporum f. sp. lycopersici (FOL) and tomato Solanum lycopersicum are controlled by three avirulence genes (AVR1-3) in FOL and the corresponding resistance genes (I-I3) in tomato. The three known races (1, 2 and 3) of FOL carry AVR genes in different combinations. The current model to explain the proposed order of mutations in AVR genes is: i) FOL race 2 emerged from race 1 by losing the AVR1 and thus avoiding host resistance mediated by I (the resistance gene corresponding to AVR1), and ii) race 3 emerged when race 2 sustained a point mutation in AVR2, allowing it to evade I2-mediated resistance of the host. Here, an alternative mechanism of mutation of AVR genes was determined by analyses of a race 3 isolate, KoChi-1, that we recovered from a Japanese tomato field in 2008. Although KoChi-1 is race 3, it has an AVR1 gene that is truncated by the transposon Hormin, which belongs to the hAT family. This provides evidence that mobile genetic elements may be one of the driving forces underlying race evolution. KoChi-1 transformants carrying a wild type AVR1 gene from race 1 lost pathogenicity to cultivars carrying I, showing that the truncated KoChi-1 avr1 is not functional. These results imply that KoChi-1 is a new race 3 biotype and propose an additional path for emergence of FOL races: Race 2 emerged from race 1 by transposon-insertion into AVR1, not by deletion of the AVR1 locus; then a point mutation in race 2 AVR2 resulted in emergence of race 3. |
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Authors:
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Keigo Inami; Chizu Yoshioka-Akiyama; Yasuaki Morita; Mutsuko Yamasaki; Tohru Teraoka; Tsutomu Arie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-08-27 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-09-06 Completed Date: 2013-02-07 Revised Date: 2013-04-02 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e44101 Citation Subset: IM |
Affiliation:
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Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Fungal
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genetics DNA Transposable Elements / genetics* Fungal Proteins / genetics, metabolism Fusarium / genetics*, isolation & purification, pathogenicity* Gene Expression Regulation, Fungal Gene Silencing* Genes, Fungal / genetics* Genetic Complementation Test Genetic Loci / genetics Japan Lycopersicon esculentum / microbiology Mutagenesis, Insertional / genetics* Phylogeny Transformation, Genetic Virulence / genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; 0/Fungal Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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