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Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
MedLine Citation:
PMID:  17248775     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The genetic maps of the fission yeast Schizosaccharomyces pombe were extended through the use of haploidization (spontaneous or induced by m-fluorophenylalanine), as well as by tetrad, random spore and mitotic analysis. A new diploidization method utilizing a meiosis-deficient mutant and improved haploidization techniques was employed. As a result of these and previous studies, 118 genetic markers have been assigned to 3 linkage groups. Centromere markers for all 3 chromosomes were identified and genetic maps containing a total of 71 genes were constructed. Our experiments indicate that 3 is very likely to be the haploid chromosome number of S. pombe .
Authors:
J Kohli; H Hottinger; P Munz; A Strauss; P Thuriaux
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genetics     Volume:  87     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1977 Nov 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-06-28     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  471-89     Citation Subset:  -    
Affiliation:
Institute of General Microbiology, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.
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